Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.4.16.2 (PCP)
 3,761 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

T lymphocyte-mediated immunity is important for resistance to Francisella tularensis. To characterize the specificity of this immunity, we used membrane proteins and two lipopolysaccharide (LPS) preparations. Both membrane proteins were heat-modifiable, as indicated by their migration in sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). One had an apparent molecular mass (Mm) of 120 kilodaltons (kDa) when solubilized in the SDS buffer at room temperature, but 17 kDa after heating. The respective values for the other protein were 35 kDa before and 40 kDa after heating. Both proteins were purified by a preparative SDS-PAGE. The LPS-containing preparations were isolated by aqueous phenol (WP) or PCP (phenol-chloroform-petroleum ether) extraction (LPS-R), and rendered protein-free by treatment with proteinase K. Lymphocytes from nine subjects immunized with a live tularemia vaccine from one to three years earlier responded specifically to both an F. tularensis whole cell antigen and the 17 kDa protein in the lymphocyte blast transformation test. By contrast, the 40 kDa protein and the two LPS preparations did not stimulate any detectable lymphocyte proliferation.
 ...
PMID:Membrane proteins of Francisella tularensis LVS differ in ability to induce proliferation of lymphocytes from tularemia-vaccinated individuals. 262 30

Presentations at the Fifth Annual Conference on HIV/AIDS Research in Winnipeg (Canada) considered the level of viral load and CD4+ counts that can be used to guide therapeutic decisions. Discussions centered on the dangers of relying too heavily on any one measurement of an individual's viral load, and other topics, such as what happens when trial dropouts all have low CD4+ counts, when to worry about patients with 650 T cells, and why multidrug therapy is the least inadequate option. Among the many other topics discussed are 1) Francis Plummer's work in tracking 1500 commercial sex workers over 10 years, indicating the resistance to HIV infection of some Kenyan women; 2) use of mail-in blood spots for early HIV diagnosis; 3) development of Thai vaccine efficacy trials starting within 2 years; and 4) problems in statistical analysis of study results. The article highlights the prediction of CMV progression, the prevalence of abnormal Pap smears in HIV-positive women, the isolation of a distinct mycobacterium (Mycobacterium genavense) that is being spread through Canada, soaring HIV risk during college spring break, rectal gonorrhea as a HIV risk factor, weight loss as a predictor of PCP, and identification of a new HIV-1 subtype in Ontario which was previously common only in several Central African countries.
 ...
PMID:Winnipeg conference. What do the numbers mean? 1136 23