Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.4.11.18 (
MAP
)
7,412
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Myosin VIIA is a motor molecule with a conserved head domain and tail region unique to
myosin VIIA
, which probably defines its unique function in vivo. In an attempt to further characterize
myosin VIIA
function we set out to identify molecule(s) that specifically associate with it. We demonstrate that 17 and 55 kDa proteins from mouse kidney and cochlea co-purify with
myosin VIIA
on affinity columns carrying immobilized anti-
myosin VIIA
antibody. N-terminal sequencing and immunoblotting analysis identified the 17 kDa protein as calmodulin, whereas MS and immunoblotting analysis identified the 55 kDa protein as microtubule-associated protein-2B (MAP-2B). Myosin VIIA can also be co-immunoprecipitated from kidney homogenate using anti-calmodulin or anti-MAP2 (recognizing isoforms 2A and 2B) antibodies, confirming the strong association between calmodulin and
myosin VIIA
and between
MAP
-2B and
myosin VIIA
. Myosin VIIA binds to calmodulin with an apparent K(d) of 10(-9) M. Scatchard analysis of the binding of
myosin VIIA
to
MAP
-2B provided evidence for two binding sites, with K(d) values of 10(-10) and 10(-9) M, which have been mapped to medial and C-terminal tail domains of
myosin VIIA
. The characterization of the interaction of calmodulin and
MAP
-2B with
myosin VIIA
provides new insights into the function of
myosin VIIA
.
...
PMID:Myosin VIIA is specifically associated with calmodulin and microtubule-associated protein-2B (MAP-2B). 1117 Nov 3
