Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.4.11.18 (
MAP
)
7,412
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Trophoblast invasion is crucial for embryo implantation and placentation. Excessive trophoblast invasion leads to hydatidiform moles and choriocarcinoma.
PPM1A
is a phosphatase which dephosphorylates and inactivates a broad range of substrates, including TGF-beta,
MAP
kinases, p38 and JNK kinase cascades, and is involved in tumor suppression. The objective of this study was to investigate the expression of
PPM1A
in normal and malignant human placenta and its role in trophoblast invasion, which shares many similarities with invasion of tumor cells. By Western blotting and immunocytochemistry, significantly higher expression of
PPM1A
in human placental villi at term was found as compared with that during the first trimester. Furthermore, the expression level of
PPM1A
protein in hydatidiform moles was lower compared with that during normal pregnancy. We further investigated the function of
PPM1A
in extravillous trophoblast cell line HTR8/SVneo. Transwell migration and Matrigel invasion assays demonstrated that
PPM1A
siRNA significantly promoted the motility and invasiveness of the cells. Gelatin zymography showed that knockdown of
PPM1A
with siRNA elevated the expression of pro-matrix metalloproteinase pro-(MMP)-9, but down-regulated tissue inhibitors of metalloproteinases (TIMP)-2. The present data indicate that
PPM1A
plays a critical role in the regulation of normal placentation by inhibiting trophoblast migration and invasion.
...
PMID:Protein phosphatase 1A (PPM1A) is involved in human cytotrophoblast cell invasion and migration. 1940 68
