Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.4.11.18 (
MAP
)
7,412
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The DNA damage response likely includes a global phosphorylation signaling cascade process for sensing the damaged DNA condition and coordinating responses to cope with and repair the perturbed cellular state. We utilized a label-free liquid chromatography-mass spectrometry approach to evaluate changes in protein phosphorylation associated with
PP5
activity during the DNA damage response. Biological replicate analyses of bleomycin-treated HeLa cells expressing either WT-
PP5
or mutant inactive
PP5
lead to the identification of six potential target proteins of
PP5
action. Four of these putative targets have been previously reported to be involved in DNA damage responses. Using phospho-site specific antibodies, we confirmed that phosphorylation of one target, ribosomal protein S6, was selectively decreased in cells overexpressing catalytically inactive
PP5
. Our findings also suggest that
PP5
may play a role in controlling translation and in regulating substrates for proline-directed kinases, such as
MAP
kinases and cyclin-dependent protein kinases that are involved in response to DNA damage.
...
PMID:Novel Ser/Thr protein phosphatase 5 (PP5) regulated targets during DNA damage identified by proteomics analysis. 2003 4
