Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.2.1.36 (hyaluronidase)
 4,606 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The ability to demonstrate AMPS in the trabecular region in the normal eye of the Rhesus monkey was shown to be critically dependent upon technical variation. Staining the fixed specimen prior to dehydration and embedding permits the uniform demonstration of AMPS in the trabecular region of the Rhesus monkey and shows it to be hyaluronidase-sensitive. Electron microscopy using the modified technique shows the reaction products to be present within the trabecular band, the intertrabecular spaces, and the canal of Schlemm. More impressive distribution was seen in the basement membrane of trabecular endothelium intimately related to the cell wall and in the ground substance and basement membrane of the endothelium of the inner wall of the canal Schlemm. The technique is also successful in the human eye and suggests a greater abundance of trabecular AMPS in open-angle glaucoma.
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PMID:Demonstration of acid mucopolysaccharides in the trabecular meshwork of the Rhesus monkey. 4 31

In 1962, when the immune complex in nephritic glomerular basement membrane (GBM) was clarified as being a type of GBM thickening, Amon and Gayer reported a different type of thickening in the rabbit administered hyaluronidase (an enzyme to degrade hyaluronic acid) and named it 'herniation' of the GBM. As we have been interested for a long time in the disappearance of normally present nonsulfated AMPS, presumably hyaluronic acid (HA), from the glomeruli in humans and experimental animals with chronic glomerulonephritis, we wanted to observe the activity of the enzyme in these conditions. Since a suitable histochemical method for the precise evaluation of hyaluronidase is unavailable, we instead chose beta-glucuronidase(beta-Gase), which is also an enzyme which degrades HA. The principal study was performed by means of light- and electron-microscopic histochemistry of chronic glomerulonephritis produced experimentally in rats and compared the obtained results to those in human chronic glomerulonephritis. The high activity of beta-Gase with a coincidental decrease of AMPS in the glomeruli was observed both in experimental and human chronic glomerulonephritis. The herniation type GBM thickening in the rat was coincidental with the enzyme localization with the disappearance of AMPS from foot processes of epithelial cells overlaying the lesion. The results might suggest the key role of beta-Gase in the deformation of GBM in chronic glomerulonephritis in general.
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PMID:Acid mucopolysaccharide and one of its glomerular degrading enzymes beta-glucuronidase in experimental and human glomerulonephritis. 617 21

Acid mucopolysaccharides in the extracellular compartment of early chick blastoderms (16 h of incubation) were labelled with tritiated glucosamine and/or [35S]sulphate. The incorporation pattern was studied autoradiographically. Treatment with testicular hyaluronidase revealed a testicular hyaluronidase-sensitive fraction, mainly at the periphery of Middle Layer and Deep Layer cells, and a testicular hyaluronidase-resistant fraction, mainly at the ventral side of the Upper Layer. A biochemical analysis, utilizing chondroitinase ABC and nitrous acid, followed by cellulose acetate electrophoresis, demonstrated the synthesis of a non-sulphated fraction, i.e. hyaluronic acid and/or chondroitin, and a sulphated fraction, comprising two undersulphated components, i.e. chondroitin sulphate, and heparan sulphate or heparin. The appearance of different AMPS in specific areas of the early chick blastoderm is regarded as an early specialization of the extracellular compartment.
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PMID:Localisation and characterization of acid mucopolysaccharides in the early chick blastoderm. 644 84