Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
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Drug
Enzyme
Compound
Query: EC:3.2.1.36 (
hyaluronidase
)
4,606
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Glucose oxidase from Aspergillus niger,
hyaluronidase
from Streptomyces hyalurolyticus, and cholesterol oxidase and
cholesterol esterase
from Pseudomonas fluorescens were effectively adsorbed on an Amberlite CG-50 column, when the cell-free cultured medium or the cultured medium with cell extract and without cell debris was applied without desalting but at pH less than or equal to 4.5. At the acidic pH, all the ion-exchange groups (-COOH) exist in the protonated form; the adsorption is not due to electrostatic attraction, but to hydrophobic interaction. The enzymes thus adsorbed were effectively eluted by increasing pH, at which the ion-exchange groups became dissociated. This type of adsorption-elution is called hydrophobic-ionic chromatography. By a single run of chromatography, glucose oxidase,
hyaluronidase
, cholesterol oxidase, and
cholesterol esterase
were purified 30-fold, 12-fold, 45-fold, and 20-fold with yields of 82%, 83%, 80%, and 90%, respectively. This indicates that hydrophobic-ionic chromatography on an Amberlite CG-50 column is effective for the purification of various enzymes, provided that they are stable at the acidic pH.
...
PMID:Hydrophobic-ionic chromatography: its application to microbial glucose oxidase, hyaluronidase, cholesterol oxidase, and cholesterol esterase. 680 71
Thyroid hormones control the levels of the lysosomal enzymes,
cholesterol esterase
and
hyaluronidase
, in rat liver, skeletal muscle and skin. Activities of these enzymes decreased to approximately 40-50% of normal levels in liver cell fractions, skeletal muscle homogenates and skin homogenates from thyroidectomized rats. Treatment of hypophysectomized rats with L-triiodothyronine increased the activities of
cholesterol esterase
and
hyaluronidase
approximately 2-fold in liver cell fractions. The low levels of
hyaluronidase
and
cholesterol esterase
in thyroidectomized rats may account for the accumulation of mucopolysaccharides in skin and for the elevation of serum cholesterol levels in hypothyroid patients.
...
PMID:A possible explanation of myxedema and hypercholesterolemia in hypothyroidism: control of lysosomal hyaluronidase and cholesterol esterase by thyroid hormones. 721 32
