Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.2.1.36 (hyaluronidase)
 4,606 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The primary hyperplastic nature of palisaded encapsulated neuromas (PENs) has been recently challenged by suggesting a traumatic origin. We studied eight cases of traumatic neuroma (TN) and 12 cases of PEN by routine light-microscopic, histochemical, and immunohistochemical methods to assess evidence of previous tissue injury. Sections from the formalin-fixed, paraffin-embedded tissue were stained with hematoxylin-eosin, trichrome, elastic, reticulin, Giemsa, colloidal iron (with and without hyaluronidase), and Bielschowsky silver stains. Antibodies were applied to collagen types I, III, and IV, MAC 387, factor XIIIa, alpha 1-antitrypsin (A1AT), epithelial membrane antigen (EMA), Leu-7, and myelin basic protein using ABC techniques. We found that (a) in TN the individual fascicles were usually surrounded by perineurial cells, whereas in PEN the perineurial cells were observed mainly in the capsular areas and only rarely within the fascicles as evidenced by EMA antibodies; (b) histochemically TN contained considerably larger amounts of collagen (types I and III), acidic mucin, and myelin products than did PEN; and (c) neither PEN nor TN contained increased inflammatory cells or cells positive for factor XIIIa, MAC 387, or A1AT. We conclude that (a) there are substantial structural and histochemical differences between TN and PEN, (b) the changes suggest that the classic form of PEN has a different histogenesis than TN, and (c) on histologic grounds, chronic minor trauma could not be excluded as an etiologic factor for PEN.
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PMID:Comparative light-microscopic and immunohistochemical study of traumatic and palisaded encapsulated neuromas of the skin. 128 69

A cell line (NMSG10) was established from malignant fibrous histiocytoma in human tibial bone. The cells revealed polymorphism at the primary culture stage, but they gradually became monotonous fibroblastic cells during transfer. On light microscopic examination, the features of these transformed cells were shown to be positive for alcian blue, oil red 0 and acid phosphatase stain. In an immunohistochemical test, the cells were shown to be positive for anti-proteoglycans, S-100, alpha 1-antitrypsin, vimentin and actin. An electron microscopic examination, revealed multiple irregular long microvilli extending from the cell surface. Also noted were microvesicles and lipid vacuoles in the cytoplasm. There was also a collagenous microfilament in the extracellular matrix. Furthermore, we found a lot of mucous substance which was able to be stained with alcian blue and to be digested by hyaluronidase in the cultured medium. This mucous substance was identified through two dimensional electrophoresis as being mainly hyaluronic acid with a macromolecular weight, a small amount of heparan sulfate and chondroitin sulfate. Additionally, chromosomal analysis was performed at the 7th passage, revealing aneuploidy with a modal number of 46. The primary cells and NMSG10 cells were not transferable to nude mice.
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PMID:[Establishment and characterization of NMSG10 cells from malignant fibrous histiocytoma in human bones]. 196 90