Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.2.1.36 (
hyaluronidase
)
4,606
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The extracellular matrix (ECM) acts as a critical factor during morphogenesis. Because the organization of the ECM directly influences the structure of tissues and organs, a determination of the way that ECM organization is regulated should help to clarify morphogenesis. We have analyzed the assembly of
Del1
, an ECM protein produced by endothelial cells in embryos, in the ECM.
Del1
consists of three epidermal growth factor repeats (E1-E3) at its N-terminus and two discoidin domains (C1, C2) at its C-terminus. Experiments with various deletion mutants of
Del1
have revealed that fragments containing the C-terminus of C1, which has a lectin-like structure, direct deposition in the ECM. The efficiency of deposition varies according to the presence of other domains in
Del1
. A fragment containing E3 and C1 has the strongest deposition activity, whereas fragments containing C2, which is highly homologous to C1, have low deposition activity. Digestion of ECM with
hyaluronidase
from bovine testis releases
Del1
from the ECM, suggesting that glycosaminoglycans are involved in the deposition of
Del1
. In vivo gene transfer experiments have shown that fusion with the deposition domain of
Del1
dramatically alters the distribution of exogenous proteins in mice. Thus, the extent of
Del1
deposition may modify the organization of the ECM.
...
PMID:Discoidin domain of Del1 protein contributes to its deposition in the extracellular matrix. 1770 Dec 20
