Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.2.1.36 (
hyaluronidase
)
4,606
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
A high molecular weight antifertility factor (
AF-1
) was obtained in a high degree of purity from human seminal plasma by ultracentrifugation, CM-cellulose and concanavalin A chromatography, and Sepharose 6B gel filtration. A final purification step involving preparative disc electrophoresis was occasionally required.
AF-1
showed a dose-dependent inhibition of the in vitro fertilizing ability of capacitated mouse spermatozoa, causing 50% inhibition of fertilization at approximately 27.5 micrograms/10(6) spermatozoa. Removal of the follicle cell layer of the oocyte did not decrease the antifertility effect of
AF-1
but inhibition of fertilization was no longer observed after dispersal of the zona pellucida. The effect of
AF-1
was on the spermatozoa and not on the oocytes. These results show that
AF-1
treatment prevents capacitated spermatozoa from penetrating the zona pellucida and possibly the follicle cell layer. The mechanism by which
AF-1
does so is not known because
AF-1
did not prevent the in vitro acrosome reaction of guinea pig spermatozoa, nor did it inhibit the activity of human acrosin or bovine testicular
hyaluronidase
. The antifertility activity of
AF-1
is reversed after recapacitation of the
AF-1
treated spermatozoa and it can be assumed that
AF-1
is either dissipated or loses activity during the transport of the spermatozoa through the female genital tract when capacitation takes place.
AF-1
is heat labile. The properties of
AF-1
are the same as those found previously for the pellet obtained by high-speed centrifugation of human seminal plasma, indicating that this is the primary, nonparticulate, high molecular weight factor with antifertility activity in human semen.
...
PMID:Properties of a highly purified antifertility factor from human seminal plasma. 715 55
