Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.2.1.36 (
hyaluronidase
)
4,606
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Basic fibroblast growth factor (
FGF-2
) can enhance biological potentials of periodontal ligament cells and its topical application induces considerable periodontal tissue regeneration in vivo. In this study, we examined the effect of
FGF-2
on the production of hyaluronan (HA), an extracellular matrix playing important roles in homeostasis and inflammatory/wound healing responses, by human periodontal ligament (HPDL) cells. An inhibition binding-protein assay revealed that
FGF-2
significantly increased HA production by HPDL cells in a dose dependent manner. Analysis by HPLC revealed that in conditioned medium of
FGF-2
-treated HPDL cells HA had a higher molecular mass, compared to that of untreated HPDL cells. RT-PCR analysis revealed the enhancement of mRNA expression of hyaluronan synthase (HAS) 1 and HAS 2, both of which contribute to the production of HA with a high molecular mass, but not HAS 3 in the
FGF-2
-treated HPDL cells. In contrast, three isoforms of
hyaluronidase
(HYAL) transcript were unchanged in the
FGF-2
-treated HPDL cells. These results provide new evidence for the possible involvement of
FGF-2
in the regulation of HA production and its appreciable roles in not only homeostasis but also regeneration of periodontal tissues.
...
PMID:Fibroblast growth factor-2 regulates the synthesis of hyaluronan by human periodontal ligament cells. 1557 76
