Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.2.1.36 (
hyaluronidase
)
4,606
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Epigallocatechin gallate
(
EGCG
) is a catechin and an abundant polyphenol in green tea. Although several papers have evaluated
EGCG
as a cosmetic constituent, the skin hydration effect of
EGCG
is poorly understood. We aimed to investigate the mechanism by which
EGCG
promotes skin hydration by measuring
hyaluronic acid synthase
(
HAS
) and
hyaluronidase
(
HYAL
) gene expression and antioxidant and anti-pigmentation properties using cell proliferation assay, Western blotting analysis, luciferase assay, 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, and reverse transcription polymerase chain reaction (RT-PCR) analysis. RT-PCR showed that
EGCG
increased the expression of natural moisturizing factor-related genes
filaggrin
(
FLG
),
transglutaminase-1
,
HAS-1
, and
HAS-2
. Under UVB irradiation conditions, the expression level of
HYAL
was decreased in HaCaT cells. Furthermore, we confirmed the antioxidant activity of
EGCG
and also showed a preventive effect against radical-evoked apoptosis by downregulation of caspase-8 and -3 in HaCaT cells.
EGCG
reduced melanin secretion and production in melanoma cells. Together, these results suggest that
EGCG
might be used as a cosmetic ingredient with positive effects on skin hydration, moisture retention, and wrinkle formation, in addition to radical scavenging activity and reduction of melanin generation.
...
PMID:Skin Protective Effect of Epigallocatechin Gallate. 2931 35
