Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.2.1.36 (hyaluronidase)
 4,606 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The effect of exploitation and adrenergic system drugs on sexual reflexes and properties of the semen in somatically mature Polish Large White boars has been presented. At the first stage 8 males were exploited twice a week. Then, previous to semen collection, the animals were given beta-adrenolytic (Propranolol--Polfa), after 30 minutes followed by the preparation Levonor--Polfa (alpha-adrenomimetic). 15 minutes after last injection the semen was collected, this procedure having been repeated twice a week. At the second stage of the experiment the same males were exploited once a week. Like in the first stage, the animals were given the same doses of the drugs in the same sequence. 15 minutes after last injection the semen was collected once a week. At the third stage of the experiment the boars were exploited every day. Again, like in the previous stages, the animals were given the above mentioned beta-adrenolytic and alpha-adrenomimetic to collect the semen 15 minutes after last injection. In all the stages of the experiment 6-8 ejaculates were collected from each boar. Apart from preliminary assessment of concentration and morphology of spermatozoons there was determined the level of protein and activity of selected enzymes in the semen. The sexual drive as well as the time of searching reflex and ejaculation were observed. The drugs applied have been proved to be able to prolong the time of ejaculation reflex. Simultaneous application of beta-adrenolytic and alpha-adrenomimetic to animals exploited twice a week appeared to increase the number of spermatozoons in the whole ejaculate, the percentage of spermatozoons with proper movement and the activity of GGT and fucosidase. Intensive exploitation of a boar with parallel application of the preparations described bring about a gradual decrease in spermatozoons and protein in the ejaculate as well as in the activity of hyaluronidase in its plasma and in conversion to mg protein of semen plasma.
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PMID:[Effect of adrenergic drugs and exploitation on sexual reflexes and semen properties in Polish large white boars aged 2-3 years]. 257 39

The characteristics of alpha-adrenoceptors in rat myocardium were investigated by specific binding of [3H]prazosin to cells isolated from adult rat heart by perfusion with collagenase and hyaluronidase. The cells were incubated in Krebs-Ringer bicarbonate buffer gassed with 95% O2 and 5% CO2 at 31 degrees with the appropriate concentrations of the different ligands. Non-specific binding was defined by the addition of 10(-5) mole/l. phentolamine. The binding of [3H]prazosin was saturable and reached equilibrium within 15 min. Scatchard analysis showed a straight line giving an apparent dissociation constant, Kd, equal to 155.9 +/- 8.0 pmole/l. and a maximal number of binding sites equal to 76.7 +/- 11.1 fmole/mg protein. Inhibition of specific [3H]prazosin binding by different adrenergic blockers showed the order of potency characteristic of alpha 1-adrenoceptors: prazosin much greater than phentolamine greater than yohimbine much greater than propranolol. Inhibition by adrenergic agonists showed the order of potency: adrenaline greater than noradrenaline = phenylephrine greater than isoprenaline. The same orders of potency were observed in the presence of propranolol. However, propranolol slightly decreased the affinity for noradrenaline and phenylephrine. Hofstee analyses of the inhibition curves showed two binding components for all ordinary alpha-adrenoceptor blockers and agonists including unlabelled prazosin. In contrast, [3H]prazosin showed only one binding component. Both binding components were of the alpha 1-adrenoceptor subtype according to the order of potency of blockers. The different ligands had different affinity ratios for the two binding components giving them different profiles. Trifluoperazine, a phenothiazine compound, also had high affinity for the [3H]prazosin binding sites. This drug, however, apparently detected one class of binding sites only, as interpreted from the Hofstee analysis. Hill analyses of the inhibition data consistently yielded Hill constants, nH, in the range 0.75-0.85 except for [3H]prazosin, where nH = 1.02 and for trifluoperazine, where nH = 1.07. Although the two binding components may serve different functions, it seems impossible at present to relate the negative and the positive inotropic components, respectively, of the alpha-adrenergic inotropic response observed in functional studies only to one or the other binding component.
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PMID:Specific binding of [3H]prazosin to myocardial cells isolated from adult rats. 632 25

The mechanism for reduced myocardial ischaemic injury by hyaluronidase was studied in open chest anaesthetized dogs. Repeated coronary artery occlusions were performed and the effect of hyaluronidase (225 NF units per kg) was studied during infusion of noradrenaline 0.125 mg/kg . min. Ischaemic injury was measured as the sum of ST-segment elevations (sigma ST) at 10-15 sites. Regional myocardial blood flow was determined by tracer microspheres. Blood for metabolic studies was sampled from a local coronary vein draining ischaemic tissue and from the coronary sinus draining predominantly non-ischaemic tissue. Hyaluronidase reduced sigma ST and increased subepicardial and transmural blood flow in ischaemic myocardium, but flow was not significantly changed in the ischaemic subendocardium or in non-ischaemic myocardium. Hyaluronidase had no significant effect on arterio-local venous differences of oxygen, glucose, lactate or free fatty acids across the ischaemic myocardium. In conclusion, reduction of myocardial ischaemic injury by hyaluronidase can be explained by increased collateral blood flow and not by an effect on fluxes of substrates across the ischaemic myocardium.
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PMID:Effect of hyaluronidase on substrate exchange and blood flow in the ischaemic myocardium of the dog. 720 7