Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.2.1.36 (hyaluronidase)
 4,606 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Scanning electron microscopy permits three-dimensional morphological study of the outer layer of the synovial membrane. This article concerns the study of 15 surgical samples of normal and pathological human synovial membranes: 3 normal synovial membranes, 6 inflammatory synovial membranes, 5 arthrosic synovial membranes, 1 tuberculous tenosynovitis. The samples were washed, fixed in a mixture of glutaraldehyde and iso-osmotic paraformaldehyde, dehydrated in increasing concentrations of alcohol and at the critical point, and then subjected to double metallization (aluminium and gold). Certain portions were treated with hyaluronidase. Hypertrophy of the villi, considerable in inflammatory synovial membranes, minimal in arthrosic synovial membranes, and absent in the normal synovial membrane, was confirmed. The outer layer is composed of cells of different sizes with spaces betwen, in which images indicating intercellular bridges can just be seen. The surface of the cells is irregular and covered with numerous microvilli. The arthrosic synovial membrane is poorly with large cells; in contrast in an inflammatory synovial membrane they are numerous, globular, and project from the surface.
Rev Rhum Mal Osteoartic 1976 Jan
PMID:[Contribution of scanning electron microscopy to the study of normal and pathological human synovial membrane]. 125 85

This study examines the contribution of hyaluronan, a rich anionic glycoprotein, to angiotensin-II-induced contraction (AII: 10(-11) to 10(-8) M) of endothelium-free strips of aorta, mesenteric artery and vein obtained from normal rabbits. Tissues are treated with hyaluronidase (HYAL: 1 mg/ml) during 60 min before being mounted in organ baths superfused with normal Krebs solution for isotonic contraction. Isotonic contraction of the mesenteric artery to the four highest doses of AII is reduced by 50 to 60% following HYAL treatment, compared to the normal contraction curve (0.01 < p < 0.001). Isotonic contraction of the aorta and mesenteric vein to AII is not influenced by HYAL. Isometric contraction curves of the three tissues to AII are not modified by HYAL. In additional experiments, the Krebs solution was selectively enriched in calcium (3.8 mM/l) and in sodium (160 mEq/l) to verify if the effect of HYAL is associated with interstitial washing in the concentration of these cations, because of the hyaluronan digestion. In fact, the calcium-rich superfusion is associated with complete correction of the HYAL-induced reduction of the mesenteric artery isotonic contraction. The sodium-rich superfusion failed to normalize the depressed mesenteric artery contraction. Since HYAL only affected isotonic contraction of the resistance artery (mesenteric), it is likely that the interstitial space of this tissue contains more hyaluronan than the aortic or mesenteric vein matrix, or that HYAL only affected the smooth muscle cell population involved in the circular tonus of the resistance vessel. Correction of the abnormality by calcium enrichment of the Krebs solution suggests that a relative diminution and/or a redistribution of this important cation, obtained following the interstitial degradation of hyaluronan.
Arch Mal Coeur Vaiss 1996 Aug
PMID:[Contribution of the vascular interstitial matrix to induced contraction by angiotensin II. In vitro studies on the rabbit aorta, mesenteric artery and vein]. 894 67