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Enzyme
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Target Concepts:
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Query: EC:3.2.1.36 (
hyaluronidase
)
4,606
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Ovulated rat oocytes are activated spontaneously soon after recovery from the oviducts. To investigate the kinetics and mechanism of rat oocyte spontaneous activation (OSA), we investigated the effect of aging in oviducts,
hyaluronidase
treatment, and extracellular and intracellular calcium, and examined the activity of
CaMKII
and the effect of its inhibitor on OSA. Oocyte aging in oviducts and
hyaluronidase
did not affect OSA. However, OSA was significantly decreased in calcium-free medium and in calcium-containing medium containing L-type calcium channel blocker and IP(3)R inhibitor. Moreover, significantly lower OSA was shown with an inhibitor of
CaMKII
. There was a significant increase of
CaMKII
activity at 30min after oocyte recovery and constitutively active
CaMKII
was located near the meiotic spindle in freshly recovered oocytes. Therefore,
CaMKII
is one of the upstream signals to activate rat oocytes spontaneously after recovery and rat oocytes respond very sensitively to extracellular calcium.
...
PMID:Extracellular calcium induces activation of Ca(2+)/calmodulin-dependent protein kinase II and mediates spontaneous activation in rat oocytes. 1757 Mar 44
