Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:3.2.1.36 (hyaluronidase)
 4,606 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

1. The effect of vasoactive intestinal polypeptide (VIP) upon adenylate cyclase activity was determined in purified cortical basolateral membranes and in glomeruli and tubular elements obtained from rabbit kidney. 2. In purified basolateral membranes prepared from cortex, 1 microM-VIP consistently stimulated adenylate cyclase activity above basal levels (1.55 +/- 0.09-fold (mean +/- S.E. of mean), n = 10 animals). Half-maximal stimulation was observed at 17 +/- 11 nM-VIP (S.D., n = 9). 3. Related peptides, e.g. secretin, glucagon, gastric inhibitory peptide, human pancreatic growth hormone releasing factor, and peptide having N-terminal histidine and C-terminal isoleucine amide (PHI), were without effect or gave lower stimulations of adenylate cyclase activity when tested at 1 microM. 4. Significant VIP degradation was observed under the assay conditions used but this did not substantially alter the response or selectivity to VIP. 5. In separate preparations of isolated glomeruli and proximal tubules addition of 1 microM-VIP resulted in a 3.3 +/- 1.1-fold (S.D., n = 3) and 2.2 +/- 1.0-fold (S.D., n = 3) stimulation (respectively) of adenylate cyclase activity. 6. In isolated medullary tubule suspensions, isolated by collagenase-hyaluronidase digestion of outer (red) medulla, and in thick ascending-limb-enriched preparations prepared by Percoll density gradient fractionation, 1 microM-VIP significantly increased adenylate cyclase activity by 2.4 +/- 0.6-fold (S.D., n = 3) and 2.1 +/- 0.7-fold (S.D., n = 3) respectively. 7. A possible role for VIP in the regulation of renal function in the rabbit is discussed in relation to the occurrence of VIP stimulation of adenylate cyclase activity in several renal cellular elements.
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PMID:Vasoactive intestinal polypeptide regulation of rabbit renal adenylate cyclase activity in vitro. 365 72

We have previously shown that a protein-independent growing fibrosarcoma, Gc-4 PF has a high motile response to its cultured medium, which is associated with an increase in expression of gp78, a cell surface receptor for autocrine motility factor (AMF). Here we show that the cultured medium contains two motile activities, acidic and basic AMFs with regard to binding features on ion exchange chromatography. These two AMFs were purified by sequential DEAE anion exchange, CM cation exchange, and gel filtration chromatographies. However, both acidic and basic AMFs have a similar size of 55 kDa and 65 kDa under non-reducing and reducing conditions, respectively, with the same pI of 6.5. The stimulated motility of both AMFs was inhibited by the pertussis toxin (PT), but not by Streptomyces hyaluronidase. These two AMFs significantly stimulated the lung colonizing properties of the self-producing cells by 1.5-fold. These results suggest that both acidic and basic AMFs may correspond to the previously reported AMF and confirm directly that the AMF-gp78 signaling pathway is involved in cell motility associated with metastatic property.
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PMID:Differential purification of autocrine motility factor derived from a murine protein-free fibrosarcoma. 830 29

The oocytes of females from the inbred mouse strains KE (albino) and CBA (agouti) differ in the following characteristics: the appearance of the cytoplasm is clear in KE but granular in CBA; the cumulus oophorus dispersion with hyaluronidase is quick in KE but slow in CBA; dissolution of the zona pellucida with a proteolytic enzyme is slow in KE but quick in CBA; and the maturation stage at the time of ovulation is metaphase I in KE while in CBA the first polar body has been extruded. Aggregation chimaeras were produced to investigate whether these differences are intrinsic or extrinsic to developing oocytes. Among 13 fertile chimaeric females, two produced only CBA oocytes, four produced both CBA and KE oocytes and were germline chimaeras, and seven produced only KE oocytes, as recognized by progeny testing. The larger number of females producing KE oocytes is a result of strong selection against CBA germ cells. All chimaeric females had cumuli composed of both KE and CBA cells, as recognized by the time of dispersion and glucose phosphate isomerase electrophoresis, but the presence of non-homologous cumulus cells did not change the character of the developing oocytes significantly. The conclusion from this study is that the rate of meiotic maturation, sensitivity of oocyte investments to enzymes, and deposition of granules in ooplasm are determined largely autonomously by genes acting in the germ cells.
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PMID:Phenotype and maturation rate of oocytes of chimaeric mice produced from two strains that differ in oocyte quality. 915 37