Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.2.1.36 (hyaluronidase)
 4,606 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The effect of antiserum raised against rat urinary (renal) hyaluronidase has been examined in rats subjected to antidiuretic stimuli (water-deprivation or vasopressin infusion). Prior administration of antiserum abolishes the reduction in medullary and papillary extractable hexosamine which normally accompanies antidiuresis. Antiserum against rat testicular hyaluronidase was found to be without effect during water-deprivation. Water-loading significantly increased the level of extractable hexosamine. The findings are considered in relation to previous observations on the effects of antisera on renal and urinary composition and collecting duct morphology under identical experimental conditions. It is suggested that a functional relationship exists between the net degradation of medullary mucopolysaccharides by hyaluronidase and the concentrating capacity of the kidney.
 ...
PMID:Renal medullary hexosamine content following antidiuresis and water-loading in the rat. Effects of antisera against rat urinary and testicular hyaluronidase. 719 63

1. The influence of urinary hyaluronidase (believed to be predominantly of renal origin) on the urinary concentrating process has been studied in rats subjected to antidiuretic stimulus. 2. Antiserum against a partially purified preparation of this enzyme has been raised in rabbits. Urinary volume, solute excretion and medullary composition have been investigated in rats treated with this antiserum (0.2 ml./100 g body weight, i.v.) before water deprivation for 48 hr or infusion for up to 4 hr with arginine-vasopressin. Control rats were pre-treated with normal rabbit serum. 3. Pre-treatment with antiserum against rat urinary hyaluronidase (AUase) caused water-deprived rats to excrete urine at a rate significantly greater, and of osmolality significantly lower, than that recorded in control rats. 4. The increase in medullary solute gradient which typically accompanies antidiuresis was significantly reduced in water-deprived rats pre-treated with AUase. 5. In rats treated with AUase and infused for 4 hr with arginine-vasopressin, there was no significant increase in the medullary solute gradient, whereas this increased markedly in control rats. 6. During the first 24 hr of water deprivation there weas an increase in the rate of Ca excretion by control rats which was abolished by pre-treatment with AUase. 7. The effects of antiserum against a partially purified preparation of rat testicular hyaluronidase (ATase) were studied in water-deprived rats. No evidence was obtained that this enzyme has any influence on renal function. 8. It is concluded that urinary hyaluronidase, but not testicular hyaluronidase, plays an important role in facilitating the urinary concentrating process following antidiuretic stimulus.
 ...
PMID:The influence of hyaluronidase on urinary and renal medullary composition following antidiuretic stimulus in the rat. 726 71

Hyaluronan is a large glycosaminoglycan that is abundant in the interstitium of the renal medulla/papilla. Papillary hyaluronan increases during hydration and decreases during dehydration. Due to its gel properties and ability to retain large volumes of water, hyaluronan plays a role in renal water handling by affecting the permeability characteristics of the papillary interstitium. The focus of the present investigation was the regulation of hyaluronan metabolism in the kidney, especially during variations in hydration status. In control papillas, HAS 2 mRNA was heavily expressed and HAS 1 and 3 mRNA were weakly distributed. HYALs 1-3 mRNA were found at high expression and HYAL 4 was only weakly expressed. In hydrated animals, the diuretic response (12-fold) was followed by a 58% elevation in papillary hyaluronan and a 45% reduction in the excreted urinary hyaluronidase activity. No difference was determined in HAS 1-3 mRNA or HYAL 1, 3-4 mRNA expression, suggesting a change in activity rather than amount of protein. In dehydrated animals, antidiuresis was followed by a 22% reduction in papillary hyaluronan and a 62% elevation in excreted urinary hyaluronidase activity. Plasma vasopressin was 2.8-fold higher in dehydrated vs. hydrated rats. In conclusion, HAS 2 appears a major contributor to the baseline levels of hyaluronan. Reduced HAS 2 gene expression and increased excreted urinary hyaluronidase activity during dehydration contribute to the reduced amount of hyaluronan and to antidiuretic response.
 ...
PMID:Hyaluronan synthases and hyaluronidases in the kidney during changes in hydration status. 1963 55