Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.2.1.36 (
hyaluronidase
)
4,606
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Hyaluronic acid (HA) is a component of the extracellular matrix (ECM) that exists as a high molecular weight polymer composed of alternating disaccharides, D-glucuronic acid and N-acetyl D-glucosamine. The interaction of
hyaluronidase
with HA results in the disruption of basement membrane integrity and produces an angiogenic response that has been implicated in tumor invasiveness and metastasis. Although
hyaluronidase
is present in several neoplasms, levels of
hyaluronidase
expression in breast cancer are not known. This investigation defines the correlation of elevated levels of
hyaluronidase
with breast adenocarcinoma invasiveness. Utilizing RT-PCR, RNA was extracted from paraffin embedded tissues (n=6) of patients diagnosed with
fibrocystic breast
changes, ductal carcinoma in situ (DCIS), and invasive adenocarcinoma. After constructing cDNA primers for base pairs 504 and 759 of the PH-20 gene (which is homologous to the
hyaluronidase
gene), PCR was performed and the products were visualized with ethidium bromide using gel electrophoresis. Invasive breast adenocarcinoma had a significantly higher level of
hyaluronidase
expression compared to other breast tissue samples; (32+/-15 vs. 8+/-3; p<0.03). Elevated levels of
hyaluronidase
correlated with invasive breast adenocarcinoma. Our data suggests that elevated levels of
hyaluronidase
are associated with breast adenocarcinoma invasive potential. Hyaluronidase may play an integral role in breast cancer invasion and metastasis.
...
PMID:Association of hyaluronidase and breast adenocarcinoma invasiveness. 1020
