Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.2.1.31 (beta-glucuronidase)
 7,680 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

During 1987-1990, 62 infections with Shiga-like toxin (Verocytotoxin)-producing E. coli, other than O group 157, were identified. The isolates belonged to 26 serovars within 20 O groups; of them, 10 serovars belonged to 6 O groups of traditional enteropathogenic E. coli (EPEC). The serovars O2:H5 and O91:H14 were exclusively associated with ulcerative colitis. Eighteen patients (children under 4 years of age) developed a haemolytic uraemic syndrome. All together, 31 strains produced SLT I, 26 strains, SLT II, and 5 strains, both toxins. All strains produced beta-glucuronidase and fermented, with one exception, D-sorbitol. The phenotypic diversity of SLT-producing E. coli implies that the only means of their identification is by indirect (genetic) or direct (phenotypic) demonstration of toxin production.
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PMID:Serological and biochemical properties of Shiga-like toxin (verocytotoxin)-producing strains of Escherichia coli, other than O-group 157, from patients in Germany. 155 7

E. coli belonging to the O157 serological group are among the organisms isolated most frequently out of all the so called entero-hemorrhagic E. coli strains (EHEC). Since several years they have been isolated also in Poland. The purpose of the present study was determination on selected phenotypic and genotypic properties of E. coli O157 strains isolated in our country from clinical material samples and from food. The serotype of the strains was determined, together with the following properties regarded as pathogenicity markers of verotoxic E. coli strains such as absence of beta-glucuronidase activity and sorbitol fermentation ability, as well as production of verotoxins SLT I and/or SLT II and entero-hemolysin. Besides that, by the PCR method the fragments of the genes coding for verotoxins, intimin and enterohaemolysin were amplified. The products of PCR were analysed by the restriction enzyme analysis (RFLP). All verotoxic E. coli O157 strains isolated in Poland were analysed by the pulsed field gel electrophoresis of genomic DNA (PFGE). The studied group comprised E. coli O157 strains, among them 40 strains were isolated from human faeces and 5 from food. The remaining strains were the reference E. coli O157:H7 EDL 933 and G 5244 strains and strains from NIH collection. The obtained results showed that the tested strains were a very varying population. 21 of them (all isolated from food, 11 from faeces and 5 reference strains) belonged to serotype O157:H7, five were not peritrichous O157:NM and the remaining ones had other ciliary antigen than H7. All strains isolated from food, reference strains and only 3 O157:NM strains isolated from humans were verotoxic. The strains from food and two reference strains produced only SLT II, 2 of 3 strains isolated from humans and one reference strain also produced only SLT II and the other produced both verotoxins. Apart from these 13 verotoxic strains all remaining strains caused sorbitol fermentation.
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PMID:[Characteristics of E.coli O157 strains isolated in Poland from clinical material and food samples]. 1022 33