Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:3.2.1.31 (beta-glucuronidase)
 7,680 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

In the hippocampus of young and aged rats, lysosomal acid phosphatase and beta-glucuronidase were localized by enzyme histochemistry to the pyramidal and granule cells and to the mossy fiber zone (acid phosphatase only). Following a unilateral entorhinal lesion, enhancement in enzyme staining was observed within GFA (glial fibrillary acidic) protein-positive astrocytes and in cells resembling microglia and oligodendroglia in the ipsilateral dentate outer molecular layer and the hippocampal stratum lacunosum-moleculare. In young animals, these changes, first detected on day 2 post-lesion, were maximum on days 4 and 10 post-lesion and declined subsequently. The aged animals exhibited a delayed time-course of enzyme changes with minimal increases on days 2 and 4 post-lesion and greater increases on days 10 and 30 and gradual diminution thereafter. The delayed lysosomal response in the aged animal paralleled a reduction in the induction of glial cells following the lesion. Our findings suggest that the previously reported impairment in the clearing of degeneration debris in the aged animal is mediated by reduced glial induction and delayed glial lysosomal activation.
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PMID:Lysosomal enzyme changes in young and aged control and entorhinal-lesioned rats. 687 83

The role of dietary fibers in diabetes has been studied by several workers. Long term dietary treatment with increased amounts of fiber-rich low-glycaemic index natural foods improves blood glucose and reduces the number of hypoglycemic events in type I diabetic patients. On the other hand Rohrbach and Martin and Cohen and Surma described changes in the general and biochemical structure of renal tissues such as the glomerular basement membranes. One of these changes was the reduction and undersulfation of the glycoconjugate and glycosaminoglycan heparan sulfate, which plays an important role in renal structure and function. The purpose of the present study was to determine specific effects of two types of dietary fiber on the composition of kidney glycoconjugates in an animal model of diabetes type I. Streptozotocin-treated diabetic rats were fed either a control diet or diets containing 10% wheat bran (insoluble dietary fiber) or 5% guar gum (soluble dietary fiber). Effects of these fibers on glycaemic control and nephropathy were assessed using previously described methodologies. The effect of dietary fiber in the glycoconjugate composition of kidneys of control and diabetic animals was studied by estimating their total hexose content, sulfated glycosaminoglycans, hexosamines and uronic acids. The activities of enzymes that participate in the synthesis of saccharides and glycoconjugates (L-glutamine-fructose-6-phosphate aminotransferase) and their degradation (N-acetyl-beta-glucosaminidase and beta-glucuronidase) were also evaluated. Results indicated that both soluble and insoluble dietary fibers ameliorated a significant increase in the activity of GFAT. Heparan sulfate was also isolated and quantified. Results indicated that the renal content of heparan sulfate decreased in diabetic animals and that this decrement was ameliorated by the ingestion of both soluble and insoluble fiber in the diet.
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PMID:Dietary fibres ameliorate decreased synthesis of heparan sulphate in streptozotocin induced diabetic rats. 1277 Jun 44