Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.2.1.31 (
beta-glucuronidase
)
7,680
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Sorbitol dehydrogenase
(
SDH
) catalyses the reversible oxidation of sorbitol, xylitol and ribitol to their corresponding ketoses. In this study, we investigated the expression and role of Arabidopsis
SDH
in salt and osmotic stress tolerance, and abscisic acid (ABA) response. The expression patterns of
SDH
were investigated using transgenic Arabidopsis plants expressing
beta-glucuronidase
(GUS) under control of the promoter with the first intron of
SDH
. qRT-PCR and histochemical assay of GUS activity were used to study
SDH
expression regulation by ABA, salt and osmotic stress.
SDH
-overexpression lines of Arabidopsis were used to investigate the role of
SDH
in salt and osmotic stress, and ABA response. Arabidopsis
SDH
was predominantly expressed in source organs such as green cotyledons, fully expanded leaves and sepals, especially in vascular tissues of theses organs.
SDH
expression was inhibited by NaCl and mannitol treatments. Seed germination and post-germination growth of
SDH
-overexpressing lines exhibited decreased sensitivity to salt and osmotic stress compared to WT plants. The transcript of
SDH
was induced by ABA. Overexpression of
SDH
decreased sensitivity to ABA during seed germination and post-germination growth. Expression of AAO3 increased but ABI5 and MYB2 decreased in
SDH
-overexpressing lines after ABA treatment. This study demonstrates that expression of
SDH
is regulated by ABA, salt and osmotic stress.
SDH
functions in plant tolerance to salt and osmotic stress, and ABA response via specific regulating gene expression of ABA synthesis and signalling in Arabidopsis.
...
PMID:Overexpression of SDH confers tolerance to salt and osmotic stress, but decreases ABA sensitivity in Arabidopsis. 2912 73
