Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.2.1.31 (beta-glucuronidase)
 7,680 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The development of the filarial nematode Brugia pahangi was monitored and compared in susceptible (BLACK EYE) and refractory (ROCK) strains of Aedes aegypti. Simultaneously, the activities of acid phosphatase, beta-glucuronidase, alpha-glucosidase, and N-acetyl-beta-glucosaminidase were measured. Three- to five-day-old females of both strains were fed on infected and uninfected clawed jirds (Meriones unguiculatus) then dissected or homogenized at 2 h, at 24-h intervals for 5 days, and at 8 and 10 days after treatment. Enzyme activities were assayed by a fluorometric procedure. The susceptible strain maintained an 80% infection and 18.6 larvae/mosquito over the 10-day period. In contrast, the refractory strain was initially 33% infected and had a mean of 4.9 larvae/mosquito and this decreased to 20% by 3 days, and to 3% with a mean of 0.33 larvae/mosquito at 10 days. Significantly higher acid phosphatase and beta-glucuronidase activities were observed in the refractory strain at specific time intervals after infection. Alpha-glucosidase and N-acetyl-beta-glucosaminidase were highly variable among strains and according to infection status. Analysis of the results of this study suggests that certain acid hydrolase enzymes could be involved in the elimination of B. pahangi in refractory strains of Ae. aegypti and could be used to monitor biochemical changes in response to filarial nematode infections in certain mosquito populations.
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PMID:Comparative development of Brugia pahangi and variation in acid hydrolase enzyme titers in. 1119 15

A novel protein overexpression system of Aspergillus oryzae was constructed. Five promoters which originate from A. oryzae expressed sequence tag (EST) clones in submerged culture were obtained by genome walking. These were subjected to beta-glucuronidase (GUS) reporter assays. The promoter of manganese superoxide dismutase-encoding gene (sodM) showed the most GUS production. The sodM gene was abundantly expressed in submerged culture but little expressed in solid-state culture. The sodM promoter was approximately 3-fold induced by the addition of 0.01% H2O2. Glucoamylase production in A. oryzae using the sodM promoter led to secretion of approximately 1 g/l-broth in Czapek-Dox medium for 3 d. Fucose lectin production in A. oryzae using the sodM promoter led to overexpression as a specific and abundant intracellular protein.
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PMID:Isolation of a novel promoter for efficient protein production in Aspergillus oryzae. 1538 59