Gene/Protein
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Enzyme
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Target Concepts:
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Query: EC:3.2.1.31 (
beta-glucuronidase
)
7,680
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
In eukaryotes, histone acetyltransferases regulate the acetylation of histones and transcription factors, affecting chromatin structural organization, transcriptional regulation, and gene activation. To assess the role of
HAC1
, a gene encoding for a histone acetyltransferase in Medicago truncatula, stable transgenic lines with modified
HAC1
expression in the model plants M. truncatula, Lotus japonicus, and Arabidopsis thaliana were generated by Agrobacterium-mediated transformation and used for functional analyses. Histochemical, transcriptional, flow cytometric, and morphological analyses demonstrated the involvement of
HAC1
in plant growth and development, responses to internal stimuli, and cell cycle progression. Expression patterns of a reporter gene encoding
beta-glucuronidase
(GUS) fused to the
HAC1
promoter sequence were associated with young tissues comprised of actively dividing cells in different plant organs. The green fluorescent protein (GFP) signal, driven by the
HAC1
promoter, was detected in the nuclei and cytoplasm of root cells. Transgenic lines with
HAC1
overexpression and knockdown showed a wide range of phenotypic deviations and developmental abnormalities, which provided lines of evidence for the role of
HAC1
in plant development. Synchronization of A. thaliana root tips in a line with
HAC1
knockdown showed the involvement of this gene in the acetylation of two core histones during S phase of the plant cell cycle.
...
PMID:Different functions of the histone acetyltransferase HAC1 gene traced in the model species Medicago truncatula, Lotus japonicus and Arabidopsis thaliana. 2718 Jan 94
