Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts: Gene/Protein Disease Symptom Drug Enzyme Compound   Target Concepts: Gene/Protein Disease Symptom Drug Enzyme Compound

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Query: EC:3.2.1.31 (beta-glucuronidase)
7,680 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The following enzymatic activities were measured in serum of patients with benign and malignant ovarian tumors before treatment: alkaline and acid phosphatases, aspartyl (AspAT) and alanyl (AlAT) aminotransferases, leucyl (LAP) and alanyl (AAP) aminopeptidases, lactate dehydrogenase (LDH), gamma-glutamyl transpeptidase, cathepsin, alkaline ribonuclease (RNase) and beta-glucuronidase. It was shown that at least three determinations (phosphatases and LAP) are practically useless in a discrimination between the examined groups. RNase in combination with AspAT (AlAT) or RNase with AAP and LDH were found to give the best results as marker enzymes.
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PMID:Serum enzymes in ovarian carcinoma. 4 48

Forty strains of bacilli were isolated from flat sour evaporated milk and were characterized as Bacillus stearothermophilus (5 strains), Bacillus licheniformis (10 strains), Bacillus coagulans (15 strains), Bacillus macerans (5 strains), and Bacillus subtilis (5 strains). The hydrolases of these strains were examined with the API ZYM system, and the ability of these strains to produce acid in milk incubated at different temperatures was also examined. Esterase, esterase lipase, lipase, valine aminopeptidase, phosphoamidase, beta-glucuronidase, and beta-glucosidase were the activities found in all strains tested; they were strain-dependent and ranged between 1 and 5 by the API ZYM test. The same strains produced various concentrations of acid in milk. These organisms may be responsible for the flat sour spoilage in evaporated milk.
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PMID:Biochemical activities of Bacillus species isolated from flat sour evaporated milk. 143 Apr 75

Classification and identification of fermentative actinomycetes are labor-intensive and problematic. In this study, we evaluated the applicability and reliability of the RapID ANA II system (Innovative Diagnostic Systems, Inc., Atlanta, Ga.) and the discriminatory value of the API ZYM system (Societes Analytab Products Inc., La Balme Les Grottes, France) in the identification of Actinomyces-like bacteria by using conventional methods as a reference. Eighty-five strains, including 71 isolates from mixed anaerobic infections and 14 reference strains, were tested. The RapID ANA II system correctly identified all Actinomyces odontolyticus strains and 65% of Actinomyces israelii strains. All Arcanobacterium haemolyticum strains were misidentified as Actinomyces pyogenes. The most common isolates in the study were Actinomyces meyeri-like organisms, 84% of which, however, were aerotolerant. The identification of these aerotolerant strains thus remains unresolved and warrants further studies. New characteristics and changes to the conventional API ZYM enzyme profiles are suggested. The API ZYM enzyme profiles of A. odontolyticus and A. israelii were very similar, the only discriminating enzyme being alpha-fucosidase. In differentiation between A. pyogenes and Arcanobacterium haemolyticum, the production of beta-glucuronidase by the former and the production of acid phosphatase by the latter are suggested as new helpful characteristics for use in clinical laboratories. In summary, the RapID ANA II and API ZYM systems can be used as rapid preliminary methods in the identification of Actinomyces species but accurate identification requires supplementary conventional tests and gas-liquid chromatography.
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PMID:Evaluation of the RapID ANA II and API ZYM systems for identification of Actinomyces species from clinical specimens. 145 93

A system has been assessed for the identification of Esch. coli using a rapid triple chromogenic test which relies on the ability of the organism to produce a beta-galactosidase, a beta-glucuronidase, and indole. Coliforms which had been fully identified were tested by this system. Of 512 non-Esch. coli strains there were no false positives, whereas of 514 Esch. coli strains 486 (94.5%) were found to give positive results. Two hundred and twenty-one coliforms that had been isolated from blood cultures were also tested using the colistrip in advance of, or without knowledge of the API 20E result. The test was found to be 100% specific and 94% sensitive for the 105 Esch. coli strains. The test was rapid, simple to perform and economical.
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PMID:Escherichia coli: rapid identification by chromogenic tests. 145 2

The urinary secretion of two lysosomal enzymes, N-acetyl-D-glucosaminidase (NAG, EC 3.2.1.30) and beta-glucuronidase (GLR, EC 3.2.1.31), and two brush border enzymes, alanine aminopeptidase (AAP, EC 3.4.11.2) and gamma-glutamyltransferase (GGT, EC 2.3.2.2), was examined in apparently healthy individuals and in patients before and after renovascular surgery for treatment of hypertension. Eight out of nine patients had elevated levels of at least one enzyme before surgery. The ranking in their frequency of elevation was NAG greater than AAP greater than GLR greater than GGT. In comparing the release of any two enzymes in apparently healthy individuals, the release was coordinated except for GGT and GLR. In individual patients following surgery the excretion of the lysosomal enzymes was highly coordinated whereas the release of the brush border enzymes was less coordinated. Comparisons of lysosomal to brush border enzyme activities revealed dissimilar release patterns between these two classes of enzymes. Analysis of variance over the entire hospitalization period showed that NAG/GLR (p = 0.42) and AAP/GGT (p = 0.12) did not vary significantly whereas all comparisons of lysosomal to brush border enzymes varied significantly (p less than or equal to 0.03). These results indicate that enzymes derived from different subcellular organelles, lysosomes or brush borders, have similar release patterns. However, the lack of a significant correlation between lysosomal and brush border enzyme excretion implies that the two processes are not interdependent. These studies further suggest that the transient pathophysiological changes that occur within renal cells following renovascular surgery affect these cellular components in different ways.
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PMID:A lack of coordination in the release of urinary lysosomal and brush border enzymes following renovascular surgery. 257 67

We evaluated three fluorogenic methods (MUG; Remel, Lenexa, KS) for the rapid (less than 1 hr) identification of E. coli by detecting beta-glucuronidase activity. The methods included: direct disk inoculation test, tube test, and liquid spot reagent test. Fluorogenic tests were performed on pure cultures of lactose fermenters and compared with identification by Enterotube II (Roche Diagnostics; Nutley, NJ). Organisms yielding disparate results were further analyzed by API 20E (Analytab Products Inc.; Plainview, NY). The tube test was evaluated with isolates subcultured on both MacConkey and blood agars; the direct disk and liquid spot reagent methods were tested with isolates subcultured on blood agar only. All methods were highly specific (greater than 97%). Sensitivity of the beta-glucuronidase tests were method and media dependent, but exceeded 85% in all cases. The direct disk and tube tests also permitted detection of indole formation; results of indole testing, however, contributed little to accurately identifying E. coli.
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PMID:Rapid identification of Escherichia coli with a fluorogenic beta-glucuronidase assay. 332 92

The enzymatic activities of 53 strains of Pseudomonas cepacia were determined by using the API ZYM system. Strong alkaline phosphatase, acid phosphatase, butyrate esterase, caprylate esterase, myristate lipase, leucine arylamidase, and phosphoamidase activities were consistently detected in all strains. Weak activities were observed for valine arylamidase, beta-glucosidase, and N-acetyl-beta-glucosaminidase. No activities could be demonstrated for cystine arylamidase, trypsin, chymotrypsin, alpha-galactosidase, beta-galactosidase, beta-glucuronidase, alpha-glucosidase, alpha-mannosidase, and alpha-fucosidase. Enzymatic activities of pseudomonads may provide useful information about their pathogenesis and information for identification of Pseudomonas species.
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PMID:Enzymatic characterization of Pseudomonas cepacia by API ZYM profile. 335 98

Most flavobacteria tested with the fluorogenic substrate 4-methylumbelliferyl-beta-D-glucuronide possessed beta-glucuronidase (GUD), but when some of the same strains were tested with the API ZYM gallery, all were negative for GUD. Conflicting reports also appear in the literature about starch hydrolysis among flavobacteria. We observed that the results obtained can depend on the medium used and the length of incubation. Our results indicate that GUD activity and starch hydrolysis are more widely distributed in the genus Flavobacterium than previously reported.
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PMID:A note on starch hydrolysis and beta-glucuronidase activity among flavobacteria. 380 62

Renal toxicity of aminoglycosides seems to be less frequent in newborn infants compared to adults even though glomerular filtration rate and tubular secretion and reabsorption mechanisms are subjected to adaptive processes during the neonatal period. In 14 infants, kinetic parameters of gentamicin were determined using an open three-compartment body model. According to the lower glomerular filtration rate, the beta-elimination phase is longer in the newborn infant compared to adults, while the gamma-elimination phase is quite similar to adult values. The calculated drug accumulation in the deep compartment (kidney) under steady-state conditions is lower in newborns compared to infants. The excretion of urinary enzymes of tubular origin, that is the lysosomal NAG (N-acetyl-beta-D-glucosaminidase), beta-glucuronidase, and the brush-border-associated AAP (alanine-aminopeptidase), GGT (gamma-glutamyl-transpeptidase), are lower in healthy newborn infants compared to older ones. The increase of AAP, for instance, during aminoglycoside therapy is less pronounced in newborn infants, especially in prematures, if compared to adult values. After end of therapy the AAP excretion decreases to normal. The calculated rate of this decrease takes place in a fashion similar to the release of drugs from the kidney (gamma-elimination phase). The data indicate that there may be a lower renal accumulation of aminoglycosides in newborn infants, which can be explained by the morphometric and functional characteristics of the newborn kidney.
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PMID:Renal toxicity of aminoglycosides in the neonatal period. 614 23

Clostridium perfringens was isolated from bile in 13 cases of 150 patients examined. The serotypes of C perfringens strains isolated from bile and faeces were investigated using antisera to Hobbs' type 1-17. Two or more serological types were often found in a single specimen, but in the same patient the serotypes of C perfringens strains isolated from the bile were identical with those from the faeces. Beta-glucuronidase production in these C perfringens serotypes was tested with the API-Strep system. Strains agglutinated with Hobbs' antisera produced beta-glucuronidase, but non-agglutinated strains did not.
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PMID:Clostridium perfringens and other anaerobes isolated from bile. 629 84


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