Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:3.2.1.31 (beta-glucuronidase)
 7,680 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Efficient establishment of the symbiosis between rhizobia and their host plants requires precise regulation of bacterial nod genes. The nod gene transcripts in Rhizobium meliloti have approximately 200 nucleotides of untranslated sequence 5' of the start codon (5' UTR). We measured the significance of this region by constructing fusions between deletion derivatives of nodF and the reporter beta-glucuronidase (GUS). Flavonoid-inducible expression of the fusions in R. meliloti was evident when extra copies of the positive transcriptional activators NodD1, NodD3, or SyrM were present. The fusions responded normally over a range of inducer concentrations in Rhizobium leguminosarum bv. trifolii. GUS assays in planta showed no significant difference between the deletion constructs and a wild-type fusion. We conclude that the 5' UTRs of the nod gene transcripts are unlikely to have a significant regulatory role.
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PMID:Deletion analysis of the 5' untranslated region of the Rhizobium meliloti nodF gene. 896 36

Flavonoid glycosides were metabolized to phenolic acids via aglycones by human intestinal microflora producing alpha-rhamnosidase, exo-beta-glucosidase, endo-beta-glucosidase and/or beta-glucuronidase. Rutin, hesperidin, naringin and poncirin were transformed to their aglycones by the bacteria producing alpha-rhamnosidase and beta-glucosidase or endo-beta-glucosidase, and baicalin, puerarin and daidzin were transformed to their aglycones by the bacteria producing beta-glucuronidase, C-glycosidase and beta-glycosidase, respectively. Anti-platelet activity and cytotoxicity of the metabolites of flavonoid glycosides by human intestinal bacteria were more effective than those of the parental compounds. 3,4-Dihydroxyphenylacetic acid and 4-hydroxyl-phenylacetic acid were more effective than rutin and quercetin on anti-platelet aggregation activity. 2,4,6-Trihydroxybenzaldehyde, quercetin and ponciretin were more effective than rutin and ponciretin on the cytotoxicity for tumor cell lines. We insist that these flavonoid glycosides should be natural prodrugs.
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PMID:Intestinal bacterial metabolism of flavonoids and its relation to some biological activities. 987 9