Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:3.2.1.31 (beta-glucuronidase)
 7,680 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

1. Two acid phosphatases (beta-glycerophosphatase and phenylphosphatase), acid beta-glucuronidase and cathepsin were demonstrated in the 0.25m-sucrose homogenates from whole calf thyroid tissue and from isolated calf thyroid cells. 2. The main kinetic characters of these enzymes were studied. 3. All these acid hydrolases are partially sedimentable and display a latency that is unmasked by treatment with Triton X-100 and on dilution in hypo-osmotic media. It is concluded that these acid hydrolases belong to the lysosomes.
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PMID:Lysosomal hydrolases in calf thyroid. 596 51

The purpose of this study was to isolate distinct populations of canine neutrophil granules and to compare them with neutrophil granules from other species. Size, shape, density, and content of canine neutrophil granules were determined. Neutrophils obtained by Ficoll-Hypaque sedimentation were homogenized, and granule populations were separated by isopycnic centrifugation on a linear sucrose gradient (rho, 1.14 to 1.22 g/ml). The most dense granule population (rho, 1.197 g/ml) contained all of the myeloperoxidase, beta-glucuronidase, and elastase, more than half of the acid beta-glycerophosphatase, and most of the lysozyme. The population with intermediate density (rho, 1.179 g/ml) contained lactoferrin, vitamin B12-binding protein, and the remainder of the acid beta-glycerophosphatase and lysozyme. The least dense granule population did not contain a major peak of any of the enzymes or binding proteins tested but was distinguished by density and morphology. The size and shape of the granules were determined from scanning electron micrographs and assessment of shape was aided by transmission electron micrographs. By these methods three populations of canine neutrophil granules were characterized and named: myeloperoxidase granules, vitamin B12-binding protein granules, and low-density granules.
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PMID:Characterization of canine neutrophil granules. 629 95

1. Nine acid hydrolases, cytochrome oxidase, alkaline phenylphosphatase and catalase were demonstrated in 0.25m-sucrose homogenates of newborn-rat calvaria. The acid hydrolases were: acid phenylphosphatase, acid beta-glycerophosphatase, beta-glucuronidase, beta-N-acetylglucosaminidase (beta-N-acetylaminodeoxyglucosidase), acid ribonuclease and acid deoxyribonuclease, showing optimum activity at about pH5; cathepsin, beta-galactosidase and hyaluronidase, with optimum activity at about pH3.6. 2. The main kinetic characters of these enzymes have been studied and methods for their quantitative assay have been worked out. The activities present in bone are given and compared with those found in liver. 3. Acid-phosphatase activity was assayed with phenyl phosphate and beta-glycerophosphate as substrates: activities with these two substrates appeared to be due to two different enzymes. Acid phenylphosphatase is particularly labile and is readily inactivated by various physical or chemical agents.
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PMID:Studies on bone enzymes. The assay of acid hydrolases and other enzymes in bone tissue. 1674 42

Lysosomal changes of mouse skeletal muscle during the repair of exercise injuries were studied with biochemical, histochemical, and electron microscopic methods. Treadmill running for 4 hours and 9 hours increased the activities of cathepsin C and beta-glucuronidase, but not that of beta-glycerophosphatase in mouse quadriceps femoris muscle. The highest activities occurred 3 days after exertion and were higher after the longer duration of exertion. Similar changes that were highly correlated with the activities of lysosomal enzymes occurred in the activities of glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase and in the concentration of DNA. The activities of lysosomal enzymes correlated significantly with the severity of histopathologic injuries. Histochemical stainings of beta-N-acetylglucosaminidase and beta-glucuronidase showed a strong increase in the staining intensity 3 and 5 days after exertion, both in inflammatory phagocytes and in surviving muscle fibers in the injured area, and staining intensities increased in parallel with the severity of injuries. Electron microscopy showed an increased number of autophagic vacuoles, lysosome-like bodies, and Golgi complexes in the fibers adjacent to necrotic foci, coinciding with the highest histochemical staining pattern. Lysosomal changes in surviving muscle fibers in close proximity to injured muscle fibers could, by autophagic degradation, provide structural elements for the regeneration of injured muscle fibers.
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PMID:Lysosomal changes in mouse skeletal muscle during the repair of exercise injuries. 1675 92


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