Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.2.1.31 (beta-glucuronidase)
 7,680 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The betaine aldehyde dehydrogenase (AcBADH) gene of the halophyte Atriplex centralasiatica Iljin is induced by drought, salinity, cold stress and abscisic acid, in parallel with an increase in betaine level. In order to study the molecular basis of its expression and to obtain an effective stress-induced promoter, the 5' flanking region of betaine aldehyde dehydrogenase gene (about 1.2 kb) was isolated from the halophyte A. centralasiatica Iljin by screening the genomic library. The transcription start site, which localized at 84 bases upstream of the start ATG, was determined by primer extension and 5'-RACE method. To investigate the molecular mechanism of the stress-induced gene regulation, the AcBADH promoter-beta-glucuronidase chimeric gene constructs containing six deletions were introduced into tobacco by Agrobacterium-mediated transformation. The AcBADH 5'-flanking region, a promoter strongly induced by salt stress, contains two salt-responsive enhancer regions localized between -1115 and -890, -462 and -230 and one silencer region between -890 and -641.
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PMID:Isolating the promoter of a stress-induced gene encoding betaine aldehyde dehydrogenase from the halophyte Atriplex centralasiatica Iljin. 1235 36

A 1,993 bp region upstream of the gene encoding the betaine aldehyde dehydrogenase (BADH) was isolated from Suaeda liaotungensis K., and the analysis of the promoter sequence has revealed the existence of several putative cis-elements by the PLACE database. In this study, according to the characteristic of the BADH promoter, five chimeric constructs varied in the length of promoter fragments from -1,993, -1,466, -1,084, -573 and -300 to +62 bp relative to the transcriptional start site were placed to the upstream of the beta-glucuronidase (GUS) coding region and transferred to Nicotiana tabacum L.cv.89 by Agrobacterium tumefaciens-mediated leaf-disc transformation. The functional properties of each promoter fragment were examined by GUS histochemical staining and fluorescence quantitative analyses in the transgenic tobacco leaves treated with different NaCl concentrations for 48 h. The results show that healthy transgenic plants had decreased GUS activity in leaves, whereas a higher GUS activity was observed when the transgenic plants were challenged with sodium chloride (NaCl). Induction levels were proportional to the concentration of NaCl treatment, allowing fine-tuning of protein expression. GUS enzyme activity was enhanced 6.3-fold in transgenic tobacco leaves containing -300 bp promoter fragment in the presence of 400 mmol/l NaCl compared to the noninductive leaves. This suggests that the smallest promoter fragment (-300 to +62 bp) possesses all the essential cis-acting elements and is sufficient for NaCl induction.
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PMID:Functional analysis of BADH gene promoter from Suaeda liaotungensis K. 1792 16