Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.2.1.31 (beta-glucuronidase)
 7,680 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Peripheral blood leukocytes from fifteen patients with atopic dermatitis and ten normal nonatopic volunteers were incubated with various stimuli in vitro, and the release of the lysosomal beta-glucuronidase into the supernatant was measured. beta-Glucuronidase release was significantly reduced in patients with severe atopic dermatitis after stimulation with aggregated IgG, horse antihuman lymphocyte globulin (ALG), zymosan, and yeast-activated serum. There was an indirect correlation (r = -0.83) between aggregated IgG-induced beta-glucuronidase release and the intensity of clinical symptoms; however, there was no correlation with serum IgE levels. The enzyme release measured was not caused by cellular lysis, except for high concentrations of antilymphocyte globulin, as determined by lactic dehydrogenase (LDH) activity in the supernatant. It is concluded that lysosomal enzyme release defects might be involved in the well-known decreased resistance to infections in patients with atopic dermatitis.
J Am Acad Dermatol 1983 Mar
PMID:Decreased release of lysosomal enzymes from peripheral leukocytes of patients with atopic dermatitis. 683 38

Neutrophils and monocytes from patients with necrobiosis lipoidica (NL) and granuloma annulare (GA) were studied in vitro in order to detect functional abnormality. Phagocytosis of latex particles and zymosan-induced release of beta-glucuronidase were similar in patients and controls. Plasma from these patients did not enhance or inhibit phagocytosis or enzyme release from normal cells. Our studies suggest that peripheral blood leucocyte function in patients with GA and NL is normal, but this does not exclude a functional abnormality in the lesions.
Arch Dermatol Res 1983
PMID:Polymorphonuclear and mononuclear leucocyte function in necrobiosis lipoidica and granuloma annulare. 684 43

Chemotactic activities of circulating polymorphonuclear leukocytes (PMN) were determined in twenty patients with psoriasis and twenty healthy control persons. After serial dilution of the complement split product C5a and the formylated tripeptide f-met-leu-phe (FMLP), chemotaxis profiles showed that PMN migration toward both chemotaxins was significantly increased in psoriasis. In addition, PMN from psoriatic patients responded to chemotaxins at much lower concentrations compared with controls. The liberation of (lysosomal) beta-glucuronidase was also determined in cytochalasin B-treated cells confronted with increased concentrations of the chemotaxins. Secretion of this marker enzyme started at lower concentrations in PMN derived from psoriatic patients. Our observations demonstrate migratory and secretory hyper-responsiveness of PMN from psoriatic patients. This may play a role in perpetuating the psoriatic tissue reaction.
Br J Dermatol 1983 Jul
PMID:Altered polymorphonuclear leukocyte responses in psoriasis: chemotaxis and degranulation. 686 May 65

The diameters of Sudan Black B stained sebum vesicles and acid hematein stained perinuclear granules, and the numerical density of the latter, were determined in mature sebaceous preputial cells from normal male and female rats, testosterone-treated female rats and estradiol-treated male rats. Statistical analysis by Student's t-test showed that the diameter of lipid droplets was significantly higher in male than in female controls and that testosterone increased female values up to make control levels. Estradiol treatment decreased male values to levels below those of normal male and female controls and of testosterone-treated female rats. Diameters of perinuclear granules did not vary among animal groups but their numerical density was larger in testosterone treated than in normal female rats or estradiol treated male rats. Lipid droplet sizes and perinuclear granule numbers are thus increased by androgens and decreased by estrogens, which was interpreted an meaning that sexual hormones do not only act on sebaceous cell multiplication or turnover time as previously known, but also on the production of lipid and the output of beta-glucuronidase containing granules in this gland.
Arch Dermatol Res 1981
PMID:The effect of sexual hormones on the lipid and proteinaceous secretion of the rat preputial sebaceous gland. 727 16

The lysosomal enzymes, acid-phosphatase and beta-glucuronidase, were released from rat liver lysosome when exposed to 400 nm irradiation in the presence of haematoporphyrin, and the release was prevented by adding vitamin E, diazabicyclo-octane, bovine serum albumin, superoxide dismutase or D-mannitol to the reaction mixture. Monochromatic irradiation with wavelengths from 380 to 410 nm caused no significant differences in the release of lysosomal enzymes, but 420 nm irradiation caused three-fifths of that of 400 nm irradiation. The malondialdehyde level in rat liver homogenate increased after 400 nm irradiation in the presence of haematoporphyrin. Reduction of nitroblue-tetrazolium was not observed when haematoporphyrin was excited by 400 nm; it was considered that superoxide anion radical (O2--) was not primarily generated. The following mechanism was assumed: that porphyrin which had been excited by 400 nm, converted ground-state molecular oxygen (3O2) to excited singlet oxygen (1O2), which formed lipid peroxides in lysosomal membrane resulting in destruction of the membrane; skin changes would occur from these released lysosomal enzymes.
Br J Dermatol 1980 Jan
PMID:Lysosome destruction and lipoperoxide formation due to active oxygen generated from haematoporphyrin and UV irradiation. 737 79

In the paper presented here the following parameters on the skin of pure-bred Wistar inbred rats were investigated during the early postnatal maturation period: DNA-content (according to Burton 1956), nitrogen-content (according to Strauch 1965), beta-glucuronidase activity (according to Fishman 1974), partly also protein-content (according to Lowry 1951). At the same time, we tested if a single i.p. injection of 6-methyl-prednisolone, applied exactly at the same time of the day 24 h preceding the killing, has an influence on the above mentioned quantities in comparison to the control group (possibly dosage-dependent). In the observation period (2nd, 4th, 6th, 9th, and 14th day) a linear, statistically significant increase of the nitrogen-content could be stated on the skin. After an initial increase up to the 4th day of life the DNA-content significantly decreases during the following postnatal maturation period until the 14th day. These results could be obtained both in the control groups, and in the animals treated with different dosages of 6-methyl-prednisolone. It is of importance that these parameters (especially the cell and the DNA-content respectively) were not statistically significantly changed by pretreatment with the glucocorticoid at any day investigated. The total activity of the further tested lysosomal indicator-enzyme beta-glucuronidase increases during the postnatal maturation period up to its maximum on the 9th day of life before it decreases till the 14th day. The applied dosages of the glucucorticoid led to a general increase of the enzyme activity, which, however, was only exceptionally statistically significant. This enzyme induction could also by discussed in terms of a maturation acceleration. Dosage-dependent differences could partly be demonstrated.
Arch Dermatol Res 1980
PMID:[The influence of a single glucocorticoid administration on the DNA and the nitrogen-content as well as on the beta-glucuronidase activity of rat skin during postnatal development (author's transl)]. 745 7

Circulating human neutrophils from patients with severe inflammatory disorders such as erysipelas and sepsis are specifically desensitized to complement factor C5a stimulation but not to stimulation with other stimuli like N-formyl-methionyl-leucyl-phenylalanine (FMLP), interleukin-8 (IL-8), leukotriene B4 (LTB4), or platelet-activating factor (PAF, 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine). In this study, we raised the question whether factors released from polymorphonuclear leukocytes (PMNs) can specifically down-regulate C5a-dependent neutrophil functions. When neutrophils were preincubated with either neutrophil lysates or neutrophil degranulation supernatants, a complete inhibition of C5a-stimulated beta-glucuronidase release and chemotaxis could be observed, whereas FMLP-, IL-8-, LTB4- or PAF-dependent functions were not affected. Serine protease inhibitors like phenylmethylsulfonyl fluoride, antileukoprotease, or elafin abolished this effect. High-performance liquid chromatography of neutrophil degranulation supernatants revealed pronounced inhibition of C5a-dependent neutrophil functions in fractions exerting elastase or cathepsin G activity, but not in fractions exerting proteinase 3 activity. Using purified human leukocyte elastase (HLE), C5a responses like intracellular calcium influx, beta-glucuronidase release, and chemotaxis were also specifically inhibited. Our experiments show that the release of HLE or cathepsin G from neutrophils specifically down-regulates the responsiveness of neutrophils to C5a. Elastase and cathepsin G may therefore play an important role in the down-regulation of acute inflammation.
Exp Dermatol 2004 May
PMID:Human leukocyte elastase and cathepsin G are specific inhibitors of C5a-dependent neutrophil enzyme release and chemotaxis. 1514 22

The abdominal skin of guinea pigs was irradiated by a Xenon short arc lamp with glass filters. Acid phosphatase and beta-glucuronidase activity increased in the irradiated skin. The change of acid phosphatase is slow and mild in intensity, while that of beta-glucuronidase is quick and high in degree. The activity of acid phosphatase is influenced equally by light with filters of UV-25, UV-27, UV-29, and UV-31, while that of beta-glucuronidase is affected more mildly by light with a UV-31 filter than with any of the other three filters. These findings suggest that there is a close relationship between cutaneous changes induced by ultraviolet light and changes in lysosome activity, and that energy required to induce changes in enzyme activity is much less than energy required to produce erythema.
J Dermatol 1976 Apr
PMID:Ultraviolet light and lysosome activity. 1563 46


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