Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.2.1.31 (beta-glucuronidase)
 7,680 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Monocytes derived from the peripheral blood of patients with familial Mediterranean fever (FMF) demonstrated a consistently lower phagocytic capacity (38-44%) for 125I-labelled Shigella flexneri when compared to monocytes from healthy subjects. Phagocytosis of both viable and killed Staphylococcus albus was similar in patients and controls. However, FMF monocytes had a two- to eight-fold depressed bactericidal capacity against S. albus in comparison to normal monocytes. Acid phosphatase and beta-glucuronidase monocyte activities were similar in patients and controls. It is suggested that the defects in monocyte function may be of importance in the pathogenesis of FMF. Colchicine had no effect on any of the indices studied.
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PMID:Monocyte function in familial Mediterranean fever. 34 7

Macrophages were obtained by pulmonary lavage from normal rabbits or rabbits that had developed pulmonary granulomas after receiving intravenous BCG vaccine 2-3 weeks earlier. The cells were disrupted in iso-osmotic sucrose and a low-speed supernatant was fractionated by isopycnic centrifugation on a linear sucrose density gradient. Three populations of hydrolase-containing granules (putative lysosomes) were found in both normal and BCG-induced macrophages. They were distinguished by their different distributions in the gradient and different sensitivities to disruption by digitonin and were termed:type A, containing lysozyme; type B, containing N-acetyl-beta-glucosaminidase, beta-glactosidase, beta-glucuronidase and possibly some lysozyme; type C, containing cathepsin D. Acid phosphatase appeared to be about equally distributed between type B and C granules. Type A and B granules from BCG-induced macrophages showed markedly greater equilibrium density than did those from normal macrophages. Beta-glucuronidase and acid phosphatase had greater specific activity in the induced cells.
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PMID:Analytical subcellular fractionation of alveolar macrophages from normal and BCG-vaccinated rabbits with particular reference to heterogeneity of hydrolase-containing granules. 45 80

Acid phosphatase and beta-glucuronidase were used as marker enzymes of lysosome, and their role in adjuvant-induced uveitis was studied. These enzyme activities in the iris-ciliary processes were increased in the inflamed tissues. Changes in these enzyme activities in the tissues paralleled the development of uveitis. While protein concentration in the aqueous humor as a parameter of vascular permeability was significantly correlated with these enzyme activities, there was no correlation in the leucocyte counts in the aqueous humor. Topically applied dexamethasone reduced the increase in the aqueous protein, the leucocyte migration and the swelling of the iris-ciliary processes, while topically applied indomethacin reduced only the leucocyte migration among these inflammatory parameters. Acid phosphatase activities in the inflamed tissues were reduced also by dexamethasone, but not by indomethacin.
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PMID:[The role of lysosomal enzymes in adjuvant-induced uveitis in rabbits (author's transl)]. 53 21

Cytochemical investigations performed in 24 men with precancerous states of the larynx, i.e. leukoplakia, papillomas and pachydermia, indicated that the peripheral blood neutrophils from these patients exhibit a significant intracellular deficiency of beta-glucuronidase activity and of total lipids, as well as an increased alkaline phosphatase activity. Acid phosphatase and N-acetyl-beta-glucosaminidase activities did not differ significantly between patients and normal controls. In the authors' opinion, the neutrophil beta-glucuronidase deficiency might be a specific disturbance of neutrophils in the precancerous states and the cancer of the larynx. The possible significance of this disturbance and the subsequent decrease of antitumor immune reactivity are discussed.
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PMID:The enzymatic equipment of neutrophils in patients with precancerous states of the larynx. 63 1

The chronic Erysipelas-polyarthritis in pigs has been considered an animal model resembling human rheumatoid arthritis. Fifteen specifically pathogenfree (SPF) pigs 45 days old were experimentally infectec with strain T 28 of Erysipelothrix rhusiopathiae-bacteria. During the subsequent 32 weeks several enzymatic, immunohistological and microbiological parameters were monitored. Compared to 5 age and sex matched healthy controls the infected pigs showed increased activity of plasma acid phosphatase starting 4 weeks after the infection. Acid phosphatase activity was usually enhanced in synovial fluid of chronically ill animals. Histochemically increased activity of acid phosphatase, beta-glucuronidase and beta-acetylglucosaminidase was found in lining cells and fibroblasts of the synovial membrane of chronically diseased joints. Immunohistochemically Erysipelas-antigen was demonstrated in the synovial membrane even of those inflamed joints from which no living bacteria had been isolated. The microbiological and immunohistochemical results correlated positively with the enzymehistochemical data. The release of lysosomal enzymes from cells of the synovial membrane in chronically diseased joints due to the influence of Erysipelas-bacteria and the possible implications of persistent bacteria on the perpetuation of chronic Erysipelas-polyarthritis are discussed.
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PMID:[Enzyme, enzyme-histochemical and immunohistological studies in chronic erysipelas polyarthritis of swine]. 63 67

Distribution of acid phosphatase, beta-glucuronidase, and lysosomal hyaluronidase in the anterior segment of the rabbit eye was studied biochemically. Acid phosphatase activity was higher in the anterior uvea and cornea but lower in the sclera. Beta-Glucuronidase activity was higher in the anterior uvea but lower in the corneoscleral tissues. Lysosomal hyaluronidase activity was higher in the anterior uvea. The inner layer of the corneoscleral junction showed the highest specific activity of beta-glucuronidase and lysosomal hyaluronidase among the corneoscleral tissues. Lysosomal hyaluronidase activity was detected in all corneoscleral tissues.
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PMID:Distribution of acid phosphatase, beta-glucuronidase, and lysosomal hyaluronidase in the anterior segment of the rabbit eye. 70 Sep 53

The biochemical correlates of droplet formation in renal inner medullary cells of potassium-deficient rats were studied. An increase in the activities of five hydrolytic enzymes typical of lysosomes was associated with an increase in the number and size of droplets observed during progressive potassium depletion. Acid phosphatase activity increased 7-fold whereas beta-glucuronidase, beta-galactosidase, cathepsin, and acid DNase increased 2- to 4-fold in medullary homogenates at 25 days of depletion. Following potassium repletion the activities returned to normal at a rate dependent upon the duration of potassium depletion. The decreases in enzyme activities were associated with a concomitant rapid disappearance of the droplets from medullary cells. Protein synthesis for new droplet enzyme formation was studied by measuring the rate of [14C]leucine incorporation into protein in medullary slices. The rate increased at 1 day of depletion and reached a maximum which was 139 per cent higher than control after 7 days of depletion. In droplets isolated from medullary tissue during progressive potassium depletion the rate of protein labeling with [14C]leucine and acid phosphatase specific activity increased in parallel. When droplet proteins were separated by gel electrophoresis, acid phosphatase activity was detected in a protein band which had been labeled with [14C]leucine, thereby suggesting new enzyme protein formation. The increase in enzyme and protein synthesis and a previously demonstrated increase in phospholipid synthesis and membrane formation indicate that potassium depletion induces specific alterations in renal inner medullary cell metabolism which result in increased lysosome formation.
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PMID:Formation of renal medullary lysosomes during potassium depletion nephropathy. 83 28

The activities of acid phosphatase, beta-glucuronidase and N-acetyl-beta-glucosaminidase were measured in blood plasma, afferent and efferent popliteal lymph, intestinal lymph and in four different organs of the sheep. Acid phosphatase had an optimum activity at pH 4-5 while beta-glucuronidase and N-acetyl-beta-glucosaminidase had optimal activities at pH 5-0 and pH 4-5-5-0, respectively. Comparative studies showed that the sheep had very low activities of acid phosphatase and relatively low activities of N-acetyl-beta-glucosaminidase in the blood plasma compartment in relation to the activities of these enzymes in the plasma of the rabbit and the rat. The tissue activities of all three enzymes were relatively high when compared with those in the two non-ruminant species. It is considered that the low plasma activities of acid phosphatase in the sheep reflect a rapid turnover of this enzyme in the plasma compartment. The activities of the three enzymes in regional lymph indicated that acid phosphatase was being added to the capillary filtrate at a regional tissue level, whereas beta-glucuronidase and N-acetyl-beta-glucosaminidase in lymph were derived from filtration from the blood plasma compartment on a molecular weight basis. The high lymph: plasma ratios observed for acid phosphatase activity in intestinal lymph may indicate a function of this enzyme in lipid absorption in the sheep.
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PMID:Lysosomal enzyme activities in sheep plasma and lymph. 87 Sep 61

The activities of acid phosphatase and beta-glucuronidase were assayed in fetal and neonatal mouse epidermis by microanalytical methods. The level of acid phosphatase activity was low in the epidermis on Day 15 of gestation. Acid phosphatase activity increased 10-fold between Day 15 of gestation and neonatalhood. On the other hand, beta-glucuronidase activity was high on Days 14 and 15 of gestation and low after Day 17 of gestation. The relative ratio of acid phosphatase to beta-glucuronidase activity appeared to represent a marker for the degree of differentiation in whole epidermis.
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PMID:Developmental patterns of acid hydrolases during differentiation of fetal mouse skin. 98 92

The distribution of acid phosphatase, beta-N-acetylglucosaminidase, beta-glucuronidase, and acid beta-galactosidase was studied in mm. extensor digitorum longus, soleus, and diaphragm of rats. Using the technic of semipermeable membranes activities of these enzymes were demonstrated beside cells of the interstitial tissue in muscle fibers themselves as well. Acid phosphatase displayed the highest activity which appeared in many small dots dispersed in the fiber. The activity of acid phosphatase was about 1.2 X higher in the m. soleus than in the m. extensor digitorum longus. In the latter muscle a somewhat higher activity was often found in muscle fibers displaying a higher staining for NADH tetrazolium reductase. The activity of beta-N-acetylglucosaminidase was slightly lower, that of beta-glucuronidase very weak but still discernible. The activity of acid beta-galactosidase was not ascertained in the majority of fibers. The ratio of activities measured in an area of the same size in cells of the interstitial tissue and in muscle fibers amounted in average to 2.6:1 in the case of acid phosphatase, 2.5:1 in the case of beta-N-acetylglucosaminidase, 5.7:1 in the case of beta-glucuronidase, and 44.3:1 in the case of acid beta-galactosidase. The importance of the histochemical technic in studies concerned with acid hydrolases in striated muscle fibers in normal and pathological conditions is pointed out.
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PMID:Histochemistry of some acid hydrolases in striated muscles of the rat. 99 74


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