Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.2.1.31 (beta-glucuronidase)
 7,680 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Chemically induced hypothyroidism changes the functions of rat alveolar macrophages. Treatment of female rats with an anti-thyroid drug, methimazole (1% aqueous solution in drinking water for 6 weeks) significantly (p less than 0.05) reduced the ability of alveolar macrophages (MAM) to phagocytose and kill the yeast, Saccharomyces cerevisiae. Undigested yeasts were observed in phagolysosomes within MAM using transmission electron microscopy. The activities of the lysosomal enzymes, acid phosphatase and beta-glucuronidase, and the Fc receptor binding ability for immunoglobulin G, were lowered in MAM when compared with control macrophages (CAM). MAM also produced less tumor necrosis factor under the stimulation of lipopolysaccharide.
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PMID:Effect of methimazole-induced hypothyroidism on alveolar macrophages. 167 73

A new method was developed for the simultaneous detection and quantitation of 6-acetyl-morphine (MAM), amphetamine, benzoylecgonine (BZE), cocaine, codeine, dihydrocodeine, EDDP (methadone metabolite), methadone and morphine in hair. The hair samples were washed, cut into 2-cm segments, pulverized, incubated with phosphate buffer and beta-glucuronidase/aryl-sulfatase. After solid phase extraction and derivatization with pentafluoropropionic anhydride/pentafluoropropanol, the drugs were identified and measured by gas chromatography/mass spectrometry using their deuterated analogues as internal standards. The method is reproducible with detection limits under 0.1 ng/mg hair for almost all substances tested. Fifteen hair samples from five subjects of a methadone treatment program were collected in a 6-month period. The hair samples were segmented and examined for methadone, its main metabolite EDDP, and drugs of abuse. Of the 96 segments analysed, 95% were positive for methadone (mean value, 10.9 ng/mg), 76% for the metabolite EDDP (mean value, 1.2 ng/mg), 69% for opiates (mean values, MAM, 7.3 ng/mg; morphine, 2.9 ng/mg; codeine, 1.0 ng/mg) and 43% for cocaine (mean values, cocaine, 2.6 ng/mg; BZE, 1.1 ng/mg). A correlation of 0.63 was found between administered methadone dosages and concentrations measured by hair analysis. Further investigation is needed to clarify interindividual differences.
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PMID:Simultaneous determination of drugs of abuse (opiates, cocaine and amphetamine) in human hair by GC/MS and its application to a methadone treatment program. 813 20