Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:3.2.1.31 (beta-glucuronidase)
 7,680 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Five new binary vectors have been constructed which have the following features: (1) different plant selectable markers including neomycin phosphotransferase (nptII), hygromycin phosphotransferase (hpt), dihydrofolate reductase (dhfr), phosphinothricin acetyl transferase (bar), and bleomycin resistance (ble); (2) selectable markers are located near the T-DNA left border and; (3) selectable marker and beta-glucuronidase (uidA) reporter genes are divergently organized for efficient expression, and can easily be removed or replaced as needed.
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PMID:New plant binary vectors with selectable markers located proximal to the left T-DNA border. 146 55

We describe a transient transfection protocol for cultured Leishmania major promastigotes, utilizing Escherichia coli genes encoding beta-galactosidase and beta-glucuronidase inserted into an expression vector derived from the dihydrofolate reductase-thymidylate synthase locus. Less than 0.1 pg of either reporter enzyme can be detected with a simple fluorimetric assay, and transfection of 10 micrograms of either reporter construct yields activities at least 100-fold over background. Simultaneous introduction of both constructs showed that the activity of each reporter gene was unaffected by the presence of the other, allowing one reporter construct to serve as a control for experimental variability in test gene constructs containing the second reporter gene. These results show that it is feasible to apply transient expression assays to the identification of cis-acting elements of genes encoding nonabundant mRNAs in the genus Leishmania.
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PMID:Simultaneous transient expression assays of the trypanosomatid parasite Leishmania using beta-galactosidase and beta-glucuronidase as reporter enzymes. 190 8

Full-length cDNA of SD strain CMV (SD-CMV) RNA 3 was cloned and sequenced. An Nsi I site was created at the sequence around the start codon of coat protein (CP) gene and a replacement cassette was constructed. The CP gene was replaced by green fluorescent protein (GFP) gene, beta-glucuronidase (GUS) gene or mouse dihydrofolate reductase (DHFR) gene, respectively. The cDNAs of Fny-CMV RNAs 1 and 2 and the chimeric SD-CMV RNA 3 were cloned between the 35S promoter and terminator separately. Tobacco protoplasts transfected with the CMV cDNA vectors expressed the three reporters, implying that CMV could be used as an expression vector.
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PMID:[Cucumber mosaic virus cDNA vector expressed foreign genes in tobacco cells]. 1133 Jan 85