Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.2.1.31 (
beta-glucuronidase
)
7,680
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
MYB proteins play important roles in the regulation of plant growth, development, and stress responses. Overexpression of
BplMYB46
from
Betula platyphylla
improved plant salt and osmotic tolerances. In the present study, the interaction of eight avian
myeloblastosis
viral oncogene homolog (MYB) transcription factors with BplMYB46 was investigated using the yeast two-hybrid system, which showed that BplMYB46 could form homodimers and heterodimers with BplMYB6, BplMYB8, BplMYB11, BplMYB12, and BplMYB13. Relative
beta-glucuronidase
activity and chromatin immunoprecipitation assays showed that the interaction between BplMYB46 and the five MYBs increased the binding of BplMYB46 to the MYBCORE motif. A subcellular localization study showed that these MYBs were all located in the nucleus. Real-time fluorescence quantitative PCR results indicated that the expressions of
BplMYB46
and the five
MYB
genes could be induced by salt and osmotic stress, and the
BplMYB46
and
BplMYB13
exhibited the most similar expression patterns.
BplMYB46
and
BplMYB13
co-overexpression in tobacco using transient transformation technology improved tobacco's tolerance to salt and osmotic stresses compared with overexpressing
BplMYB13
or
BplMYB46
alone. Taken together, these results demonstrated that BplMYB46 could interact with five other MYBs to form heterodimers that activate the transcription of target genes via an enhanced binding ability to the MYBCORE motif to mediate reactive oxygen species scavenging in response to salt and osmotic stresses.
...
PMID:BplMYB46 from
Betula platyphylla
Can Form Homodimers and Heterodimers and Is Involved in Salt and Osmotic Stresses. 3086 67
