Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:3.2.1.31 (beta-glucuronidase)
 7,680 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The cytochemical reactions of 5 acid hydrolases, alpha-naphthyl acetate esterase (ANAE), acid phosphatase (AP), beta-glucuronidase, beta-glucosaminidase and dipeptidylaminopeptidase IV (DAP IV) were investigated in lymphocytes from 30 patients with B cell chronic lymphocytic leukaemia (B-CLL). Based on ANAE and AP reactivities, 4 cytochemically distinctive subgroups were identified: Group 1: AP and ANAE less than 50% positive lymphocytes (5 cases); Group 2: AP greater than 50%, ANAE less than 50% positive lymphocytes (11 cases); Group 3: AP less than 50%, ANAE greater than 50% positive lymphocytes (7 cases); Group 4: AP and ANAE greater than 50% positive lymphocytes (7 cases). beta-Glucuronidase displayed similar patterns of reactivity to AP. beta-Glucosaminidase activity was observed in the majority of lymphocytes in most patients, whereas DAP IV activity was present in less than 20% of lymphoid cells. The study failed to establish any relationship between cytochemical grouping and patients' clinical status, peripheral lymphocyte counts, E or mouse rosette values, light or heavy chain cellular immunoglobulin (Ig) class. Attempts to correlate acid hydrolase and Ig heavy chain isotype expression, putative markers of B cell maturation, were unsuccessful and indicate that within the narrow spectrum of B cell differentiation seen in B-CLL these characteristics are unrelated.
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PMID:Acid hydrolase activities in B cell chronic lymphocytic leukaemia lymphocytes: correlation of cytochemical reactions with immunological phenotype. 285 52

In 84 leukemic non-Hodgkin-lymphomas the diagnostic evidence of peripheral blood smears in Pappenheim-staining and diverse cytochemical reactions (PAS, alpha-naphthyl acetate esterase, acid phosphatase, acid esterase, beta-glucuronidase) was analysed using a homogeneous cell grid (lymphatic differential blood picture). In all entities the small lymphocyte proved to be the most frequent cell-form. The other lymphomas of low malignity (intermediate malignity) can clearly be demarcated from the CLL by the more intense polymorphism of the blood smears and in the majority can be classified entity-related by the differential blood picture. For the diagnostic evidence of the differential blood picture proved significant that entity-typical cell-forms (e.g. lymphoplasmacytoid lymphocytes, centrocytes) following the small (mature) lymphocyte as cell-type of second frequency are flooded out into the peripheral blood in non-Hodgkin-lymphomas. Highly malignant lymphomas could clearly be demarcated from low-malignant ones by their higher proportion of blasts in the differential blood picture. Of the cytochemical reactions only the acid phosphatase, with definitely focal-perinuclear reaction, an importance as T-cell marker is ascribed.
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PMID:[Diagnostic value of the differential lymphatic blood picture in leukemic non-Hodgkin's lymphomas]. 350 Dec 7

Four acid hydrolases, acid phosphatase (AP), alpha-naphthyl acetate acid esterase (ANAE), beta-glucuronidase (BG) and N-acetyl-beta-glucosaminidase (NABG) were determined cytochemically in B-chronic lymphocytic leukaemia (B-CLL) cells exposed in vitro to the tumor promoter 12-0-tetradecanoyl phorbol 13 acetate (TPA). TPA, which has been previously shown to induce B-CLL cells to mature towards plasmacytoid cells, results in the progressive expression of the enzymes tested in the cytoplasm of malignant cells, in particular AP and ANAE. Furthermore, the sensitivity to inhibitors and the pattern of reactivity of ANAE provide evidence for an enzyme subtype normally restricted to plasma cells. Thus, acid hydrolases--some of which showing plasma cell type of activity--are expressed during B-CLL cells differentiation induced in vitro. These results confirm the value of cytochemistry in subtyping B-cell malignancies.
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PMID:Cytochemical analysis of acid hydrolases expression during phorbol diester (TPA)-driven differentiation of B-chronic lymphocytic leukaemia cells in vitro. 633 64

The ultrastructural localization of four acid hydrolases (acid phosphatase, beta-glucuronidase, beta-glucosaminidase and alpha-naphthylacetate esterase) has been studied in lymphocytes from 16 patients with three types of chronic T-cell leukaemia, namely, T-prolymphocytic leukaemia (T-PLL), T-chronic lymphocytic leukaemia (T-CLL) and adult T-cell lymphoma leukaemia (ATLL). Different patterns of enzyme distribution were observed in the leukaemic T-cells from these disorders. In T-PLL, reactivity for the four acid hydrolases was confined to single or a few large granules. Gall bodies were reactive for beta-glucuronidase, b-glucosaminidase and alpha-naphthylacetate esterase but apparently unreactive for acid phosphatase. In T-CLL, scattered small- to medium-size cytoplasmic granules and many parallel tubular arrays were strongly reactive for acid phosphatase, beta-glucuronidase and beta-glucosidase but showed no reactivity for alpha-naphthylacetate esterase. Intermediate features were observed in ATLL. The observed differences in enzyme reactivity reflect a different content of lysosomal granules in the various types of leukaemic T-cells. They also suggest that similar differences may be found in normal T-lymphocyte subsets.
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PMID:Ultrastructural cytochemistry of chronic T-cell leukaemias. A study with four acid hydrolases. 660 30

This study indicated that (i) high levels of N-acetyl-beta-glucosaminidase and beta-glucuronidase could be demonstrated cytochemically in T-CLL lymphocytes, and (ii), the biochemical activities of both enzymes were significantly increased in T-CLL lymphocytes compared with B-CLL lymphocytes. It would appear that the biochemical and cytochemical determination of these lysosomal enzyme activities offers an additional means of distinguishing T-CLL and B-CLL lymphocytes.
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PMID:Lysosomal enzyme activities in a case of T-cell chronic lymphocytic leukaemia. 697 Apr 7

Four acid hydrolases, acid phosphatase (AP), alpha-naphthyl acetate esterase (ANAE), beta-glucuronidase, and N-acetyl-beta-glucosaminidase, were determined cytochemically in peripheral blood lymphocytes from 50 patients with B and T chronic lymphocytic and prolymphocytic leukemias (CLL, PLL) and related disorders. Strong positive reactions were characteristic of the T-cell leukemias while the reactions were weak or negative in B-CLL and B-PLL. Differences in the cytochemical profile of T-CLL and T-PLL were noted. In both, beta-glucuronidase and N-acetyl-beta-glucosaminidase were positive; these enzymes are therefore good cytochemical markers of the chronic T-cell leukemias. AP and ANAE gave different results according to the disease process; AP was strong in T-CLL and variable in T-PLL, while ANAE was strongly positive in T-PLL, but weak or negative in T-CLL. The findings in T-CLL, a proliferation of T gamma lymphocytes, were similar to those of normal T gamma cells. In T-PLL, the findings did not relate to the membrane phenotype as defined by monoclonal antibodies.
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PMID:Cytochemistry of acid hydrolases in chronic B- and T-cell leukemias. 698 10