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Query: EC:3.2.1.31 (beta-glucuronidase)
 7,680 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The paper describes a number of tests for the rapid detection of glycosidases including alpha-glucosidase, beta-glucosidase, beta-glucuronidase, beta-xylosidase and alpha-fucosidase. The methods use heavy suspensions of viable but non-multiplying bacteria in a buffered solution of a chromogenic substrate. The results of the tests are readable within 4 h. The application of these tests to a collection of 633 strains of Enterobacteriaceae and Vibrionaceae demonstrates that some of the tests may be valuable additions to the present tests available for the identification of bacteria belonging to these families. beta-glucuronidase activity was observed only in strains of the Escherichia-Shigella group. 97 per cent of the Escherichia strains possessed beta-glucuronidase activity. beta-xylosidase activity was almost completely restricted to strains of the Klebsiella-Enterobacter group in addition to Yersinia strains. None of the strains possessed alpha-fucosidase activity.
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PMID:Rapid diagnosis of Enterobacteriaceae. I. Detection of bacterial glycosidases. 0 74

In order to test the usefulness of BRILA-MUG (= Fluorocult) medium (Merck) for isolation and identification of total coliforms and faecal coliforms in surface water according to the EC guidelines for bathing waters a total of 969 strains of different Enterobacteriaceae and Vibrionaceae species was examined under different culture conditions. These included 486 E. coli (reference strains of O-groups 1-170, enterotoxin and Verotoxin-producing strains), 149 Salmonella (subspecies I-VI), 92 Yersinia, 44 Shigella, 64 Vibrio, 16 Aeromonas, and 118 strains of other Enterobacteriaceae species. After 48 h incubation at 36 degrees C 372 (82.0%) of 486 E. coli showed the typical reactions of gas formation, fluorescence (beta-glucuronidase activity), and indole production. Examination of fluorescence after addition of 1 N NaOH (0.5 ml), or testing of indole production after subculture in tryptophane containing broth improved the amount of typical reactions to 434 (95.4%). Incubation at 44 degrees C for 48 h gave less favourable results as compared with that at 36 degrees C. Out of 483 strains of other species 3.9% Salmonella strains of subspecies II-IV, 6.25% Citrobacter freundii, and 50% Edwardsiella tarda strains yielded reactions typical of E. coli. Shigella and Yersinia strains occasionally produced indole or fluorescence, but never visible gas.
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PMID:[Reactions of different Enterobacteriaceae and Vibrionaceae species in BRILA-MUG (Fluorocult) bouillion]. 208 Sep 70

The concentrations of seven types of microorganism in the milk produced by farms using high quality milking procedures were monitored monthly. The most commonly encountered bacterium was Pseudomonas, whose concentration varied greatly between samples, but lactococci, lactobacilli and yeasts were also present at more stable concentrations. Staphylococcus aureus and beta-glucuronidase-positive Escherichia coli were occasionally detected. Listeria monocytogenes was found in the samples from only one farm, while Yersinia enterocolitica was never detected. There were seasonal variations in the concentrations of lactobacilli and yeasts over the 2 years monitored. The changes in certain bacteria (Lactococcus, Lactobacillus) on any given farm were very similar from one year to the next. The microbiological characteristics of the milk from the farms examined seemed to be fairly constant. The profiles of some bacteria used for cheesemaking were relatively stable over time. It thus seems that the milk provided by different producers could be typed.
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PMID:Monitoring the microbiology of high quality milk by monthly sampling over 2 years. 916 19