Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.2.1.31 (beta-glucuronidase)
 7,680 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Seven genes encoding putative nuclear LIM interactor-interacting factors (NIFs) from Phytophthora infestans were characterized. Four exhibited spore-specific expression and clustered within 20kb, and three hyphal forms were also identified. Pi-nifS mRNA accumulated during sporulation, and Pi-nifC1, Pi-nifC2, and Pi-nifC3 mRNA during zoosporogenesis. By expressing fusions with the beta-glucuronidase reporter gene in transformants, promoter fragments determining patterns of transcription specific to sporangia formation or zoosporogenesis were identified. Such studies also indicated that Pi-nifS was expressed at a late stage of sporulation. Pi-nifC1, Pi-nifC2, and Pi-nifC3 induction involved inositol-trisphosphate signaling based on studies with 2-aminoethoxydiphenylborate and U-73122, was independent of verapamil-sensitive signals, and required the same cool temperatures that trigger zoosporogenesis. In contrast, warmer temperatures favoring direct sporangial germination caused Pi-nifS mRNA to rapidly decay, while verapamil increased mRNA levels. The divergent expression and clustering of the spore-specific genes may help reveal how genomes and developmental pathways evolved within the genus.
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PMID:A cluster of NIF transcriptional regulators with divergent patterns of spore-specific expression in Phytophthora infestans. 1558 95