Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:3.2.1.31 (beta-glucuronidase)
 7,680 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Epithelial-cell enriched primary cultures have been established from rat ventral prostate (RVP). Minced ventral prostates were dissociated with 0.5% collagenase in F12K tissue culture medium containing 1% fetal bovine serum. This treatment resulted in the gradual removal of stromal elements from the base of the epithelial cells. After 60 minutes of digestion the aggregates of epithelial cells were washed and plated at high density in F12K plus 10% horse serum. After 48 hours in vitro the unattached cells were removed from the culture dishes, washed, and reinoculated into new culture vessels containing fresh medium. After 96 hours in vitro, the aggregates had attached to the culture vessels and spread out to yield discrete patches of epithelial cells. By 144 hours in vitro the patches of cells had grown and coalesced to form a semi-confluent monolayer of epithelial cells. Ultrastructrual examination of these cultures indicated that adjacent cells were joined by desmosomes and tight junctions and had formed "lumen-like structures" into which projected microvilli. In addition, the cells contained secretory granules and tonofilaments, giving them a morphological appearance similar to prostate epithelial cells in the intact organ. The primary cultures also retained histochemical activities for acid phosphatase, beta-glucuronidase, and succinic dehydrogenase that were similar to the intact organ.
Anat Rec 1980 Jun
PMID:Isolation, culture and characterization of epithelial cells derived from rat ventral prostate. 625 35

The pharmacodynamics of the non-steroidal anti-inflammatory drugs flunixin, tolfenamic acid and ketoprofen were studied in calves after intravenous administration. An acute inflammatory reaction was induced in tissue cages by the intracaveal injection of the mild irritant carrageenan, and the inhibition of inflammatory mediators and enzymes was investigated. The substances measured in the exudate included the enzymes (active and total metalloproteases, serine and cysteine proteases, acid phosphatase [AP], lactate dehydrogenase [LDH] and beta-glucuronidase) and the eicosanoids (prostaglandin [PG]E2 and leukotriene [LT]B4). Studies were also made of inhibition of the synthesis of serum thromboxane (Tx)B2 ex vivo, of bradykinin-induced oedema in vivo and of the generation of superoxide anions (O2-) in vitro. None of the drugs affected the concentration of LTB4, or the activities of metalloproteases, cysteine and serine proteases, AP or LDH in the exudate. All the drugs inhibited the synthesis of serum TxB2 and exudate PGE2 and inhibited the release of beta-glucuronidase. They also decreased the oedematous response to intradermally injected bradykinin and inhibited the generation of O2- ions by neutrophils in vitro. These actions may contribute to the anti-inflammatory effects of the drugs and hence to their clinical efficacy.
Vet Rec 1995 Oct 21
PMID:Comparative pharmacodynamics of flunixin, ketoprofen and tolfenamic acid in calves. 856 Jul 1

Urinary enzyme activities of alanine aminopeptidase, gamma-glutamyl transpeptidase, alkaline phosphatase, N-acetyl-beta-D-glucosaminidase and beta-glucuronidase were determined in 15 dogs with leishmaniasis and in a group of eight normal dogs. Serum creatinine and blood urea nitrogen concentrations were also measured and renal histology was examined. All the affected dogs had renal lesions. However, no significant differences in blood urea nitrogen and creatinine concentrations were found between the control group and the affected group. The urinary enzyme activities of gamma-glutamyl transpeptidase (P < 0.01), N-acetyl-beta-D-glucosaminidase (P < 0.01) and beta-glucuronidase (P < 0.05) were significantly higher in the affected dogs. Urinary enzymes therefore seem to be a more sensitive and reliable test for assessing early renal damage in canine leishmaniasis than serum creatinine or blood urea nitrogen concentrations.
Vet Rec 1997 May 03
PMID:Enzymuria as an index of renal damage in canine leishmaniasis. 916 May 31