Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.2.1.26 (
invertase
)
4,927
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Mutations in the SEC63 gene are associated with defects in protein translocation into the endoplasmic reticulum (ER) as well as in nuclear protein localization in Saccharomyces cerevisiae. To identify proteins that might interact and/or function with SEC63p, we cloned a high copy suppressor (
HSS1
) of the temperature-sensitive lethal phenotype of the sec63-101 mutant.
HSS1
is an allele-specific sec63 suppressor that encodes an integral ER membrane glycoprotein of 206 amino acids with the N-terminus in the ER lumen and C-terminal region in the cytoplasm. Haploid strains disrupted for
HSS1
are temperature-sensitive for growth and accumulate precursor forms of Kar2p and
invertase
. The
HSS1
null allele is synthetically lethal in combination with mutations affecting ER translocation. We propose that HSS1p is important for ER translocation and interacts with previously identified components of the yeast translocation apparatus.
HSS1
is identical to SEC66, which encodes a glycoprotein complexed with SEC62p and SEC63p.
...
PMID:Suppression of a sec63 mutation identifies a novel component of the yeast endoplasmic reticulum translocation apparatus. 825 94
