Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.2.1.26 (
invertase
)
4,927
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Aspergillus niger produces extracellular
beta-fructofuranosidase
under submerged (
SmF
) and solid state fermentation (SSF) conditions. After UV mutagenesis of conidiospores of A. niger, 2-deoxyglucose (10 g/l) resistant mutants were isolated on Czapek's minimal medium containing glycerol as a carbon source and the mutants were examined for improved production of
beta-fructofuranosidase
in
SmF
and SSF conditions. One of such mutant DGRA-1 overproduced
beta-fructofuranosidase
in both
SmF
and SSF conditions. In
SmF
, the mutant DGRA-1 showed higher
beta-fructofuranosidase
productivity (110.8 U/l/hr) than the wild type (48.3 U/l/hr). While in SSF the same strain produced 322 U/l/hr of
beta-fructofuranosidase
, 2 times higher than that of wild type (154.2 U/l/hr). In
SmF
, both wild type and mutants produced relatively low level of
beta-fructofuranosidase
in medium containing sucrose with glucose than from the sucrose medium. However in SSF, the DGRA-1 mutant grown in sucrose and sucrose+ glucose did not show any difference with respect to
beta-fructofuranosidase
production. These results indicate that the catabolite repression of
beta-fructofuranosidase
synthesis is observed in
SmF
whereas in SSF such regulation was not prominent.
...
PMID:Beta-fructofuranosidase production by 2-deoxyglucose resistant mutants of aspergillus niger in submerged and solid-state fermentation. 1258 33
