Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.2.1.26 (
invertase
)
4,927
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The
ERG9
gene of Saccharomyces cerevisiae has been cloned by complementation of the erg9-1 mutation which affects
squalene synthetase
. From the 5 kb insert isolated, the functional gene has been localized on a DNA fragment of 2.5 kb. The presence of
squalene synthetase
activity in E. coli bearing the yeast DNA fragment isolated, indicates that the structural gene encoding
squalene synthetase
has been cloned. The sequence of the 2.5 kb fragment contains an open reading frame which could encode a protein of 444 amino acids with a deduced relative molecular mass of 51,600. The amino acid sequence reveals one to four potential transmembrane domains with a hydrophobic segment in the C-terminal region. The N-terminus of the deduced protein strongly resembles the signal sequence of yeast
invertase
suggesting a specific mechanism of integration into the membranes of the endoplasmic reticulum.
...
PMID:Isolation and primary structure of the ERG9 gene of Saccharomyces cerevisiae encoding squalene synthetase. 180 26
