Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.2.1.26 (
invertase
)
4,927
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Gp80, a cell-adhesion molecule in Dictyostelium discoideum, is modified by N- and O-linked oligosaccharides, and a glycosylphosphatidylinositol (GPI) anchor. To identify sequences important for the addition of these modifications to
gp80
, we created a hybrid protein in which the C-terminal 136 amino acids of yeast
invertase
were replaced by the C-terminal 110 amino acids of
gp80
. When expressed in D. discoideum, this protein (Inv-
gp80
) was not GPI-anchored and was retained in a pre-Golgi compartment. Inv-
gp80
did, however, display characteristics of a transmembrane protein, suggesting a novel mechanism for its retention. We also expressed a truncated version of the hybrid protein in which the C-terminal 22 amino acids of the Inv-
gp80
were deleted. The truncated protein (Inv-gp80stop) was O-glycosylated and secreted. These observations indicate that the hybrid protein is not abnormally folded and demonstrate the importance of the C-terminal 22 amino acids in the retention of Inv-
gp80
. Together, the data suggest that oligomerization of the protein blocks its GPI anchoring.
...
PMID:Lack of glycosyl-phosphatidylinositol anchoring leads to precursor retention by a unique mechanism in Dictyostelium discoideum. 770 55
