Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:3.2.1.26 (invertase)
 4,927 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The biological activity of crude petroleum ether extracts of Oshar (Calotropis procera); Harmal (Rhazya stricta) and Hargal (Solenostemma argel) were assessed using the 4th larval instar of cotton leafworm Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae). All extracts exhibited a significant antifeedant activity at the LC50 levels. Harmal extract deterred feeding potential of insect larvae by 52.96% but decreased to 26.76 and 18.00% for Hargal and Oshar, respectively. In nutritional assays, all plant extracts affected Growth Rate (GR mg) where Harmal caused the highest rate of efficiency and followed by Oshar against 4th larval instar fed for two days on castor leaves treated with botanical extracts (LC50) and three days on un-treated leaves after that. Hargal didn't show a significant effect on growth rate compared with un-treated larvae. The differences between Consumption Index (CI mg) of larvae treated with Hramal and Hargal after five days of feeding in comparison with un-treated larvae were significant. Efficiency of Conversion of Ingested food (ECI%) to biomass reached 31.81 +/- 1.49, 26.06 +/- 1.89 and 48.67 +/- 1.54 after five days of treatment by Oshar, Harmal and Hargal, respectively. These values were 49.61 +/- 1.72, 39.12 +/- 0.54 and 53.20 +/- 0.96 for digested food utilization (ECD%) in larvae treated with the aforementioned extracts after five days, respectively. There was a remarkable inhibitory activity of plant extracts on the digestive carbohydrate enzymes, amylase and invertase in vitro. It was noticed that Harmal had a remarkable inhibitory action causing an average of 42.58% inhibition rate on amylase and 16.27% on invertase followed by Hargel and Oshar with inhibition rates of 33.27 and 19.58% against amylase, while these values averaged 11.19 and 5.97% of inhibition in case of invertase, respectively.
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PMID:Antifeedant and toxic activity of some plant extracts against larvae of cotton leafworm Spodoptera littoralis (Lepidoptera: Noctuidae). 1909 13

The present study investigated the effect of different zinc (Zn) levels on activities of gastrointestinal digestive enzymes of growing rats. Four diets including Zn-adequate (ZA; 46 mg/kg, control), Zn-deficient (ZD; 3 mg/kg), high Zn supply (ZH; 234 mg/kg) and pair-fed in which animals received the ZA diet at restricted amounts reflecting feed intake of the ZD group were fed to rats for 5 weeks. Dietary Zn was supplemented with ZnO. The results showed that Zn deficiency resulted in decreases in body weight, while ZH supply stimulated growth. The activities of sucrase, lactase and lipase were unaffected by dietary Zn levels. Maltase activity, however, was reduced in ZD group and elevated in ZH group. Amylase and protease activities were depressed by zinc deficiency. However, rats fed the Zn-repletion diet displayed higher activity of pepsin, pancreatic amylase and protease. In particular, ZH supply did have no effect on intestinal hydrolases activities. The present study suggested that zinc deficiency impaired the activities of digestive enzymes and growth of animals. However, ZH supply might improve the digestion of nutrients via increasing activities of gastrointestinal hydrolase and probably enhanced animal health.
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PMID:Effects of zinc levels on activities of gastrointestinal enzymes in growing rats. 1917 8

Mulberry leaves treated with sub-lethal dosages (LC5, LC10 and LC20) abameetin were fed to the 5th instar larvae of silkworm (Bombyx mori L.), and the food intake and digestive enzyme activities of the larvae were studied by using gravimetric method and measuring enzyme activities. The results showed that sub-lethal dosages abameetin significantly inhibited the growth and food intake of the larvae, with their body mass and its increase rate as well as their relative growth rate being significantly lower than the control, and accompanied with the decreases of food intake, its relative consumption rate, and feces amount. The efficiency of the conversion of ingested food (ECI) and that of the conversion of digested food (EDI) also reduced, but the approximate digestibility (AD) increased significantly. The amylase and sucrase activities in the midgut of the larvae treated with abameetin decreased significantly for a longer time at the beginning, and then recovered to the same as or a higher level than the control, whereas the trehalase activity decreased significantly for a shorter time at the beginning, then increased significantly, and finally recovered to the normal. It was suggested that sub-lethal dosages abameetin had definite toxicity to the silkworm, and the toxic effect was increased with increasing dosage, which could result in the turbulence of silkworm's digestive system, and further, affect its food intake and its growth and development.
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PMID:[Effects of sub-lethal dosages abamectin on food intake and digestive enzyme activities of silkworm Bombyx mori L]. 1923 58

The bacteria capable of producing the enzymes hydrolyzing carbohydrates of various degrees of complexity (from starch to sucrose) were found to be associated with the intestinal mucosa of fishes and tegument of cestodes. Presence of the bacteria displaying the sucrase activity is especially important for macroorganisms, as bacteriogenous glucose can be used by all members of the arising community. The greatest contribution to the hydrolysis of carbohydrates (both in host and parasite) is obviously made by those microorganisms which are more closely connected with the digestive-transport surfaces and are hardly removable from the intestines by peristalsis. The levels of total amylolytic activity of bacteriogenous enzymes and activity of their a-amylase under the experimental conditions are comparable to those of the enzymes involved in membrane digestion of the host and parasite, which can be evidence of the significant contribution of enzymes produced by symbiotic microflora to the digestive processes in macroorganisms.
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PMID:[The contribution of carbohydrases produced by symbiotic microflora to the digestive processes in cestodes and their host fishes]. 1950 14

1. A method is described for the preparation of an active enzyme-containing solution of pneumococci, in which no living cells are present. These enzymes are capable of hydrolyzing sucrose, starch, and inulin. 2. The invertase and amylase of pneumococcus are active within the limits pH 5 to 8, with an optimum reaction of about pH 7. This reaction range corresponds closely with limiting hydrogen ion concentrations which define growth of the organism in the presence of carbohydrate. 3. These studies indicate that the enzymes described are not true secretory products of the living cell, but are of the nature of endoenzymes, since their activity can be demonstrated only when cell disintegration has occurred.
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PMID:STUDIES ON THE ENZYMES OF PNEUMOCOCCUS : III. CARBOHYDRATE-SPLITTING ENZYMES: INVERTASE, AMYLASE, AND INULASE. 1986 62

1. Certain enzymes of Pneumococcus are destroyed by oxidizing agents formed when sterile extracts of the cellular substances are exposed to air. The carbohydrate-hydrolyzing enzymes (sucrase, raffinase, inulase, and amylase) are the most easily inactivated under these conditions, although the bacteriolytic enzyme is also reduced in activity. Similar treatment is without effect upon the active concentration of pneumococcus lipase and peptonase. 2. The enzymes which are destroyed during the oxidation of unwashed cell extracts are themselves non-reactive with molecular oxygen. The reactions by which they are destroyed seem to represent oxidations of a type similar to those proposed in previous papers for the oxidation of hemotoxin and of hemoglobin. 3. A study has been made of the relative resistance of different pneumococcus enzymes to heat and to the action of hydrogen peroxide. 4. The various enzymes may be arranged in the same order of relative resistance whether the rating be made from the standpoint of resistance to heat or of resistance to oxidation. Nevertheless, it appears that by a proper regulation of conditions of oxidation, certain labile constituents of a mixture of cellular enzymes may be inactivated with less effect upon the activity of other constituents of the mixture than when inactivation is brought about by heat.
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PMID:STUDIES ON OXIDATION AND REDUCTION BY PNEUMOCOCCUS : VI. THE OXIDATION OF ENZYMES IN STERILE EXTRACTS OF PNEUMOCOCCUS. 1986 28

The carbohydrases (maltase, lactase, sucrase, raffinase, amylase) and lipase (tributyrinase) of the Welch bacillus retain their hydrolytic activities in sterile solutions of the bacterial cells. The demonstration of the carbohydrases and detection of their hexose products constitute experimental proof that Welch bacilli attack complex carbohydrates by way of a preliminary hydrolysis. The result of experiments on the oxidation of the enzymes show that the inhibitory effect of air upon the initiation of growth of the living Welch bacillus does not include a comparable effect upon the activity of its hydrolyzing enzymes.
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PMID:STUDIES ON BACTERIAL ENZYMES : V. THE CARBOHYDRASES AND LIPASE OF THE WELCH BACILLUS. 1986 10

The saccharogenic enzymes present in potato juice were studied. The actions were followed upon the substances present in the juice and upon added sucrose, maltose, and soluble starch. Sucrase and amylase were found to be present in the juice. No indication of a maltase was obtained. The sucrase showed optimum conditions for action at pH 4 to 5, the amylase at pH 6 to 7, both upon the starch present in the juice and upon added soluble starch. The action of a yeast sucrase preparation upon the juice showed the presence of sucrose (or raffinose) in a concentration of the order of magnitude of 1 per cent.
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PMID:STUDIES ON ENZYME ACTION : XVIII. THE SACCHAROGENIC ACTIONS OF POTATO JUICE. 1987 4

Changes in extracellular enzyme activities and microbial populations were studied during the normal composting and vermicomposting of fruitpulp, vegetable waste, groundnut husk and cowdung. The microbial numbers and their extracellular enzyme profiles showed relative variation and were found increasingly more abundant in vermicompost than in normal compost leading to the conversion of agricultural waste into value added product. In vermicompost, the maximum enzyme activities (cellulase, amylase, invertase, protease and urease) were observed during 21-35 days. The cellulase, amylase and protease activities of vermicompost reached the maximum values by 28th day of 1175, 825 microg reducing sugar g(-1) hr(-1) and 28 micro mol of aminoacid g(-1) hr(-1) of vermicompost samples respectively. Similarly the invertase and urease activities reached to peak values of 876 microg reducing sugar g(-1) hr(-1) and 197 microg NH4(+)-N g(-1) ha(-1) sample on 35th day respectively. Most of the enzymes showed correlation with change in number and types of different microbial groups like bacteria, fungi and actinomycetes during vermicomposting with maximum number of 126 x 10(6), 28 x 10(4) and 93 x 10(5) CFU g(-1) sample respectively. In contrast delayed greatest enzyme activities were observed on 42-49th day i.e., last days of normal composting. Earthworms stimulated biochemical activity and nutrient cycling by 40-45% contributing to the reduction of period of degradation of agricultural wastes resulting in maturation of vermicompost by 28th day.
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PMID:Comparative assessment in enzyme activities and microbial populations during normal and vermicomposting. 2032 98

We previously reported that rats receiving total parenteral nutrition (TPN) undergo significant pancreatic atrophy characterized by reduced total protein and digestive enzyme expression due to a lack of intestinal stimulation by nutrients (Baumler MD, Nelson DW, Ney DM, Groblewski GE. Am J Physiol Gastrointest Liver Physiol 292: G857-G866, 2007). Essentially identical results were recently reported in mice fed protein-free diets (Crozier SJ, D'Alecy LG, Ernst SA, Ginsburg LE, Williams JA. Gastroenterology 137: 1093-1101, 2009), provoking the question of whether reductions in pancreatic protein and digestive enzyme expression could be prevented by providing amino acids orally or by intravenous (IV) infusion while maintaining intestinal stimulation with fat and carbohydrate. Controlled studies were conducted in rats with IV catheters including orally fed/saline infusion or TPN-fed control rats compared with rats fed a protein-free diet, oral amino acid, or IV amino acid feeding, all with oral carbohydrate and fat. Interestingly, neither oral nor IV amino acids were sufficient to prevent the pancreatic atrophy seen for TPN controls or protein-free diets. Oral and IV amino acids partially attenuated the 75-90% reductions in pancreatic amylase and trypsinogen expression; however, values remained 50% lower than orally fed control rats. Lipase expression was more modestly reduced by a lack of dietary protein but did respond to IV amino acids. In comparison, chymotrypsinogen expression was induced nearly twofold in TPN animals but was not altered in other experimental groups compared with oral control animals. In contrast to pancreas, protein-free diets had no detectable effects on jejunal mucosal villus height, total mass, protein, DNA, or sucrase activity. These data underscore that, in the rat, intact dietary protein is essential in maintaining pancreatic growth and digestive enzyme adaptation but has surprisingly little effect on small intestinal mucosa.
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PMID:Intravenous or luminal amino acids are insufficient to maintain pancreatic growth and digestive enzyme expression in the absence of intact dietary protein. 2053 7


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