Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.2.1.26 (
invertase
)
4,927
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The
CDC25
gene product is an exchange factor for Ras proteins and it activates the Ras/cAMP pathway in the yeast Saccharomyces cerevisiae. The overexpression of the
CDC25
gene in S. cerevisiae cells causes a partial glucose-derepressed phenotype which is particularly evident for expression of
invertase
. To define domains of Cdc25 protein relevant for this derepression and to test another glucose repressed system, different to
invertase
, we have overexpressed different regions of the
CDC25
gene under the control of a GAL-promoter. We found that a derepression of both GAL regulated promoters and
invertase
was related to the overexpression of
CDC25
regions that contain a functional guanine nucleotide exchange (GEF) domain. The effect on GAL-promoters was particular evident when the
CDC25
gene was under the control of a UASgal element and operates at transcriptional level, although a moderate derepression was found also for UASgal/lacZ reporter gene. Finally, the overexpression of the GEF domain of
CDC25
also caused an increase in the expression of the GAL4 regulatory gene, while a constitutive activation of the Ras/cAMP pathway did not produce any increase in GAL4 expression. These findings indicate that the overexpression of the catalytic domain of
CDC25
gene is necessary and sufficient to give a glucose-derepression of GAL promoters and of
invertase
. They also suggest that the derepression of GAL promoters occurs through an increase of GAL4 expression in a Ras cAMP independent way.
...
PMID:The overexpression of the CDC25 gene of Saccharomyces cerevisiae causes a derepression of GAL system and an increase of GAL4 transcription. 1068 55
