Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.2.1.26 (
invertase
)
4,927
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The
invertase
productivity of Saccharomyces cerevisiae IFO 0309 protoplasts in a static culture was 35 times (extracellular) and 9 times (extracellular + intracellular) higher than those of cells. When S. cerevisiae protoplasts were immobilized in 1.0%
strontium
alginate gel as an artificial cell wall, the protoplasts could be cultivated in a shake flask without disruption, and
invertase
was secreted into the broth. However, cell wall regeneration in the immobilized protoplasts was detected at about 24 h of cultivation. This implies that prevention of cell wall regeneration is a prerequisite for long term process with protoplasts. When 0.5 microg/ml aculeacin A (inhibitor of beta-1,3 glucan synthesis) was added to the broth, active protoplasts were maintained without cell wall regeneration for more than 24 h and
invertase
was produced extracellularly. Immobilized S. cerevisiae T7 protoplasts (this strain produces twice as much
invertase
as S. cerevisiae IFO 0309) were used for
invertase
production in a bubble column reactor, and a high and stable level of
invertase
(45 U/ml) was maintained for 72 h.
...
PMID:Invertase production by Saccharomyces cerevisiae protoplasts immobilized in strontium alginate gel beads. 1623 86
