Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.2.1.26 (invertase)
 4,927 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The postnatal development of the gastric mucosa was observed in Long Evans rats. In both pyloric and fundic mucosae, the mitotic index peaked at 5 days of age and then gradually decreased to reach a constant level from 14 days of age onward (P less than 0.05), the germinal region shifted from the deeper area of the mucosae upward to the middle layer. The number of parietal cells increased gradually (P less than 0.05). Gastric pH on the mucosal surface decreased from 6 on the day of birth to about 4 at 7 days of age and to about 2 at 28 days of age. The inverse relationship between the number of parietal cells and pH value on gastric mucosa was found to be highly significant (P less than 0.01). Trehalase and sucrase activities were observed at 7 days of age and lactase and maltase activities from 0 to 14 days of age. In the pyloric mucosae, foveolae containing goblet cells which were positive for Alcian blue-PAS and HID were observed between 0 and 7 days of age. These cells disappeared by 14 days of age concurrently with the decrease in pH value and increase in the number of parietal cells.
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PMID:pH-related differentiation in the epithelia of the gastric mucosa of postnatal rats. 403 97

These studies examined the potential for reorganization and differentiation of dissociated 18-day fetal rat intestine. Cultures of trypsin-dissociated fetal intestine were maintained in vitro for 1 week on a three-dimensional matrix, then transplanted into syngeneic hosts. When harvested after 4 weeks, these transplants consistently demonstrated organotypic differentiation. Spherical structures containing crypts with frequent mitotic figures and villi lined with columnar epithelium had formed. PAS staining demonstrated positive epithelial cell brush borders, goblet cells, and luminal contents. Significant levels of the microvillus membrane enzymes lactase, sucrase, maltase, and alkaline phosphatase were present in the luminal contents. Sucrase-isomaltase, an enzyme characteristic of postweaning small intestine, was demonstrated by immunoprecipitation and SDS-PAGE. Thus, both morphological and biochemical maturation occurred in the transplants.
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PMID:Organotypic differentiation of trypsin-dissociated fetal rat intestine. 661 90

Novel composite hydrogels, poly(acrylamide)-sepiolite (PAS), poly(acrylamide/acrylic acid)-sepiolite (PAAS), and poly(acrylamide/itaconic acid)-sepiolite (PAIS) were prepared and used for the immobilization of invertase. The parameters of equilibrium swelling, diffusional exponent, and diffusion coefficient of these hydrogels were calculated from swelling experiments. Invertase was immobilized onto PAS, PAAS, and PAIS and immobilized invertases (PASI, PAASI, and PAISI) were prepared. Optimum pH values for free invertase, PASI, PAASI, and PAISI are found to be 5, 5.5, 4.5, and 6, respectively, and the optimum temperatures were 30, 50, 50, and 35 degrees C for free invertase PASI, PAASI, and PAISI. It was found that K(m) values of free invertase, PASI, PAASI, and PAISI were 11.3, 41.0, 94.5, and 56.0 mM, respectively. V(max) values were 2 mumol/min for free invertase, 8.10 mumol/min for PASI, 1.30 mumol/min for PAASI, and 0.42 mumol/min for PAISI, respectively. The invertase immobilized hydrogels showed excellent, temperature, storage, and operational stability.
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PMID:Acrylamide-sepiolite based composite hydrogels for immobilization of invertase. 1989 91

Allantoic endoderm of 3.5-day chick embryos was cultured in recombination with digestive-tract mesenchymes of 6-day chick embryos, and the differentiation of the endoderm was studied, with special attention being given to the appearance of brush-border (BB) antigens and sucrase. Irrespective of the origin of the associated digestive-tract mesenchymes, the allantoic endoderm differentiated into a columnar epithelium, expressing BB antigens and sucrase, and also into a BB antigen-negative pseudostratified or stratified epithelium of cuboidal or columnar cells with PAS or alcian blue staining in the apical portion or a BB antigen-negative stratified squamous epithelium. These results suggest that 3.5-day allantoic endoderm has the potency to differentiate into intestinal and cloacal epithelium.
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PMID:Appearance of brush-border antigens and sucrase in the allantoic endoderm cultured in recombination with digestive-tract mesenchymes. 2830 16