Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.2.1.26 (invertase)
 4,927 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Some of the cellular and molecular events involved in the normal and precocious appearance of sucrase in jejunum of infant rats have been studied. Actinomycin D has been shown to inhibit (by 79%) the rise in sucrase activity usually seen after administration of hydrocortisone to 9-day-old rats. The precocious appearance of sucrase has also been studied with respect to the cytological localization of enzyme activity in the intestinal mucosa. Tissue was sectioned in a cryostat (transverse to the villi) and sucrase was assayed in homogenates prepared from the sections. By 24 h after administration of hydrocortisone to 9-day-old animals, sucrase was detectable only at the bases of the villi. During the subsequent 72 h the enzyme activity increased and spread along the villi at a rate consistent with that of cell migration. These data have lead to the conclusion that the action of glucocorticoids on enterocytes can occur only when the cells are in their proliferative phase. An ontogenic study of the ability of hydrocortisone to elicit jejunal sucrase showed that the tissue becomes increasingly responsive to the hormone with increasing age through the first and second postnatal weeks. Various hypotheses to explain this increase have been examined.
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PMID:Studies on normal and precocious appearance of jejunal sucrase in suckling rats. 113 86

Actinomycin D affects a number of functions of the epithelial cells of the small intestine. Maltase, saccharase and lactase levels in the small intestine of hamsters treated with various dosages of actinomycin D over various periods of time, differed from those observed in control animals: administration of 0.25 micrograms/g body weight, gave rise to a statistically significant increase in the maltase and saccharase levels measured after 4 h and a statistically significant reduction in the lactase levels measured after 8 h; administration of 1.5 micrograms/g body weight reduced the activity of all three enzymes at all times post-administration, the decrease being statistically significant for maltase after 2 and 8 h.
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PMID:Action of actinomycin D on glycosidase levels in the small intestine of hamsters. 190 2

Actinomycin D, at a dose of 0.25 micrograms/g body wt, produced slight increases in intestinal enzymatic activity on hamsters. At a high dose (1.5 micrograms/g body wt), actinomycin D produced inhibition of lactase activity, whereas maltase, sucrase and alkaline phosphatase activity decreased in males and increased in females. Cycloheximide (1.5 micrograms/g body wt), produced no changes in enzymatic activity. In the male and female hamster, the different actions of the antibiotic can be explained by the variations in the cortisol release produced by stress.
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PMID:Actinomycin D and cycloheximide actions on activity of some intestinal enzymes of adult hamster. 286 61

Gibberellic acid (GA(3)) induces invertase activity within 6 hours in Avena stem segments that are incubated in the dark at 23 degrees . The maximum amount of promotion is about 5 times that of invertase activity in untreated segments. GA(3) causes significant promotion of invertase activity at concentrations as low as 3 x 10(-5) mum GA(3). The increase in invertase activity elicited by GA(3) between 3 x 10(-5) mum and 300 mum closely parallels the growth promotion that is caused by GA(3) over this concentration range. In control segments, invertase activity rises steeply during the first 6 hours of incubation, then decays slowly between 12 and 48 hours. In GA(3)-treated segments, the invertase activity also rises during the first 6 hours, parallel to that in control segments and continues to rise during the next 42 hours. These changes in invertase activity during 48-hour incubation periods do not parallel the changes in growth that occur in control and GA(3)-treated segments. Cycloheximide at 10 mug/ml abolishes all GA(3)-promoted growth and invertase activity in these segments. Actinomycin D at 40 and 80 mug/ml decreases GA(3)-promoted growth by 20% and invertase activity by 38 and 44%, respectively. The data clearly support the idea that protein synthesis is necessary for GA(3)-promoted growth and invertase activity in Avena stem segments.
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PMID:Promotion of growth and invertase activity by gibberellic Acid in developing Avena internodes. 1665 32