Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.2.1.26 (
invertase
)
4,927
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Four yeast secretion signals, the 19-amino-
acid invertase
signal sequence, the 17-amino-acid acid-phosphatase signal sequence, and the pre-sequence and prepro-sequence of prepro-alpha-factor have been used to look for the secretion of recombinant human
insulin-like growth factor 1
(
IGF1
) from Saccharomyces cerevisiae. Only the prepro-sequence, often referred to as the alpha-factor leader and consisting of an N-terminal 19-amino-acid pre-sequence or signal sequence attached to a 66-amino-acid pro-region, permits secretion of
IGF1
. The signal sequences alone do not allow the translocation of
IGF1
into the endoplasmic reticulum. This is evident from the fact that
IGF1
-like molecules, to which the signal sequences are still attached, accumulate intracellularly in the cytosol. Fusion of the pro-region of the alpha-factor leader to the C-terminus of the acid-phosphatase and
invertase
signal sequences allows
IGF1
to be secreted once again. These results reveal the essential role of the pro-region of the alpha-factor leader in the secretion of
IGF1
and indicate that it may have a function in guiding a nascent
IGF1
polypeptide to a state in which translocation can occur.
...
PMID:The pro-region of the yeast prepro-alpha-factor is essential for membrane translocation of human insulin-like growth factor 1 in vivo. 160 61
