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Query: EC:3.2.1.26 (
invertase
)
4,927
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The content of free sugars and the activities of enzymes involved in carbon metabolism-sucrose synthase, acid and
alkaline invertase
, phosphoenol
pyruvate carboxylase
, malic enzyme and isocitrate dehydrogenase were determined during seed development in mungbean pods. A decrease in carbohydrate content of pod wall from 10 to 25 days after flowering (DAF) and a concomitant increase in the seed till 20 DAF was observed. Sucrose remained the dominant soluble sugar in the pod wall and seed. In the branch of inflorescence and pod wall, the activities of sucrose metabolizing enzymes, viz. acid and
alkaline invertase
, sucrose synthase (synthesis and cleavage) and sucrose phosphate synthase were higher at 5-10 DAF, whereas in seed the maximum activities of these enzymes were observed at the time of maximum seed filling stage (10-20 DAF). High activities of sucrose synthase at the time of rapid seed filling can be correlated to its sink strength. Higher activities of phosphoenol
pyruvate carboxylase
in the branch of inflorescence and pod wall than in seed may indicate the involvement of the fruiting structure for recapturing respired CO2. High activities of isocitrate dehydrogenase and malic enzyme in the seed at the time of rapid seed filling could provide NADPH and carbon skeletons required for the synthesis of various seed reserves.
...
PMID:Ontogenic changes in enzymes of carbon metabolism in relation to carbohydrate status in developing mungbean reproductive structures. 1072 78
In the present study factors responsible for low seed biomass in wild Cicer species has been investigated. Cicer judaicum and chickpea cultivar PBG-1 were investigated to compare activities of some enzymes involved in carbon metabolism in podwall and seeds during crop development. Seed filling duration in wild species was about 15 days shorter than that of cultivated varieties due to rapid loss of moisture content and hence resulted in earlier maturity and reduced seed biomass. Longer seed filling duration appeared to be an important factor responsible for greater biomass of chickpea seeds. Because of absence of phosphoenol
pyruvate carboxylase
from 25-35 days after flowering and low sucrose synthase activities, the podwall of C. judaicum is not in a position to contribute significantly to the sink filling capacity of seeds. High
acid invertase
, low sucrose synthase activities during seed storage phase cause detrimental effect on seed filling and resulting in highly reduced sink strength and productivity of wild species. Successful transfer of stress tolerance from wild Cicer species to chickpea cultivars need to prevent the transfer of these observed unfavourable biochemical factors so that the productivity of chickpea crop remains unaffected during utilization of wild Cicer species in chickpea improvement.
...
PMID:High acid invertase activity for a prolonged period in developing seeds/podwall of wild chickpea is detrimental to seed filling. 2321 68