Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:3.2.1.26 (invertase)
 4,927 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

This study focused on the relationship between plant diversity (six species richness levels) and nutrient retention and enzyme activities associated with carbon, nitrogen and phosphorus cycling in a full-scale constructed wetland (CW) fed with post-treatment domestic wastewater. Effects of plant species richness on nutrient retention and enzyme activities were assessed using soil chemical and zymological methods, respectively. Retention of NH(4)-N and NO(3)-N in the wetland substrate increased with increasing species richness, while phosphorus retention significantly decreased under the richness level of 16 species per plot. Activities of enzymes such as dehydrogenase, beta-glucosidase, invertase, phenol oxidase, L-arsparaginase, protease and nitrate reductase, while they were affected by plant species richness, were strongly depended on the presence or absence of plants in CW substrate, while activities of enzymes such as CM-cellulase, urease and acid phosphatase were strongly depended on plant species richness. We conclude that plant species richness influenced nutrient retention and enzyme activities in the substrate in our subtropical CW; increase plant species richness in CW will likely improve the efficiency of wastewater treatment.
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PMID:Effects of plant diversity on nutrient retention and enzyme activities in a full-scale constructed wetland. 1986 27

An intra-cellular beta-glucosidase was purified to homogeneity by gel filtration, ion exchange chromatography and HPGPLC from mycelial extract of Termitomyces clypeatus in the presence of the glycosylation inhibitor 2-deoxy-d-glucose. CD spectroscopy demonstrated that the purified enzyme exhibited alpha-helical conformation. MALDI-TOF identified the enzyme's molecular weight as 6688Daltons, but SDS-PAGE and immunoblotting indicated that the enzyme formed aggregates. The enzyme also showed unique properties of co-aggregation with sucrase in the fungus. The enzyme showed around 80% stability up to 60 degrees C and residual activity was 80-100% between pH ranges 5-8. The enzyme had higher specific activity against p-nitrophenyl-d-glucopyranoside than cellobiose and HPLC showed that the enzyme possesses transglycosylation activity and synthesizes cello-oligosaccharides by addition of glucose. The enzyme will be useful in synthetic biology to produce complex bioactive glycosides and to avoid chemical hazards. This is the first report of a beta-glucosidase enzyme with such a low monomeric unit size.
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PMID:Purification and characterization of a thermostable intra-cellular beta-glucosidase with transglycosylation properties from filamentous fungus Termitomyces clypeatus. 2003

Herbal medicines have been used since prehistoric times by different cultures worldwide for the treatment of diabetes. The present investigation evaluated the effect of Ficus racemosa Linn. (Moraceae) stem bark on carbohydrate hydrolyzing enzymes, viz., porcine pancreatic alpha-amylase, rat intestinal alpha-glucosidase, sucrase, and almond beta-glucosidase, using in vitro model systems. In addition, the effect of heat treatment was also studied. Untreated F. racemosa bark (FRB) significantly inhibited (p < or = 0.05) alpha-amylase, alpha-glucosidase, beta-glucosidase, and sucrase in a dose-dependent manner. Heat treatment of the sample comparably increased alpha-amylase, alpha-glucosidase, and sucrase inhibitory activities, while a marginal decrease in beta-glucosidase inhibitory activity was observed; however, no statistical differences were noted. Untreated FRB showed IC(50) values of 0.94% and 280, 212, and 367 microg/mL for alpha-amylase, alpha-glucosidase, beta-glucosidase, and sucrase, respectively, while the IC(50) values for heat treated FRB were 0.58% and 259, 223, and 239 microg/mL, respectively. Further, a significant correlation (p < or = 0.01; r = 0.791) was observed between alpha-amylase, alpha-glucosidase, beta-glucosidase, and sucrase inhibitory activities of both untreated and heat treated FRB. The results clearly demonstrate that inhibition of carbohydrate hydrolyzing enzymes is one mechanism through which F. racemosa stem bark exerts its hypoglycemic effect in vivo. Therefore, the potential exists to explore the utilization of F. racemosa stem bark in the development of nutraceuticals and functional foods for the management of diabetes and related symptoms/disorders.
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PMID:Effect of Ficus racemosa stem bark on the activities of carbohydrate hydrolyzing enzymes: an in vitro study. 2064 93

In the present work, we studied the effectiveness of the predominant halophytes of Soliman sabkha (Tecticornia indica and Suaeda fruticosa) to promote soil biological activities and ecosystem productivity. Soil Arylsulphatese ARY, beta-glucosidase beta-GLU, phosphatase PHO, invertase INV, urease URE, and dehydogenase DES activities in Extra- and Intra-tuft halophytes and plant productivity were assessed. Results revealed a high increase of microbial community and ARY, beta-GLU, PHO, INV URE and DES activities (+298%, +400%, +800%, +350%, +320%, +25% and +759%, respectively) in Intra-tuft rhizosphere as compared to Extra-tuft one, which is likely due to the significant decrease of salinity in the rhizosphere of Tecticornia indica and Suaeda fruticosa. Both perennial plants exhibited high productivities (7.4 t dry weight ha(-1) and 2.2 t dry weight ha(-1), respectively) and Na+-hyperaccumulating capacities (0.75 t Na+ ha(-1) and 0.22 t Na+ ha(-1), respectively), reducing salt constraint and favouring soil fertility. This constitutes a promising alternative to enhance productivity in such a salt-affected biotope by offering suitable microhabitat for annual glycophytes.
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PMID:Effects of the halophytes Tecticornia indica and Suaeda fruticosa on soil enzyme activities in a Mediterranean Sabkha. 2348 95


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