Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:3.2.1.26 (invertase)
 4,927 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Fructo-oligosaccharide represent one of the major classes of bifidogenic oligosaccharides in term of their production volume, because of their favorable functional properties. These include: a/ improving the intestinal microflora; b/ relieving the constipation; c/ decreasing the total cholesterol and lipid in serum; d/ the promotion of animal growth and e/ as low calorie non-cariogenic sweeteners (Chen and Liu, 1996). FOS are manufactured by two differing processes which result in slightly different end products--using either microbial fructosyl transferase (EC 2.4.1.9) or beta-fructofuranosidase (EC 3.2.1.26) with high transfructosylation activity. The present study reports on the biosynthesis of an extracellular inulinase by yeasts from genus Kluyveromyces. The strains were isolated from different dairy products. Some of the studied strains produced large amounts of extracellular inulinase activity when grown on inulin ar sucrose as carbon source. In addition, the effect of C/N ratio on the production of extracellular inulinase was studied. Thin layer chromatography showed that inulinase from K. species 19 was capable of hydrolyzing inulin, releasing monosaccharides and oligosaccharides.
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PMID:A study of extracellular inulinase activity in strains of genus Kluyveromyces. 1595 93

Salecan is a novel exopolysaccharide produced by the strain Agrobacterium sp. ZX09, and it is composed of only glucose monomers. The unique chemical composition and excellent physicochemical properties make Salecan a promising material for applications in coagulation, lubrication, protection against acute liver injury, and alleviating constipation. In this study, we cloned the Vitreoscilla hemoglobin gene into a broad-host-range plasmid pCM158. Without antibiotic selection, there was negligible loss of the plasmid in the host Agrobacterium sp. ZX09 after one passage of cultivation. The expression of Vitreoscilla hemoglobin was demonstrated by carbon monoxide (CO) difference spectrum. The engineered strain Agrobacterium sp. ZX09 increased Salecan yield by 30%. The other physiological changes included its elevated respiration rate and cellular invertase activity.
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PMID:Vitreoscilla hemoglobin promotes Salecan production by Agrobacterium sp. ZX09. 2536 90