Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.2.1.26 (
invertase
)
4,927
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Crosslinked-poly(vinylbenzylchloride), poly(VBC), beads were prepared by suspension polymerization and poly(glycidylmethacrylate) was grafted by surface-initiated-atom radical polymerization (SI-ATRP) technique. Epoxy groups of the grafted poly(
GMA
) were reacted with hydrazine and ammonia to create an affinity binding sites. The hydrazine and amine functionalized poly(VBC-g-
GMA
) beads were used as an affinity support for adsorption of
invertase
from solution and yeast crude extract. The influence of pH, equilibrium time, ionic strength and initial
invertase
concentration on the adsorption capacities of both hydrazine and amine functionalized beads has been investigated. Maximum
invertase
adsorptions onto hydrazine and amine functionalized beads, were 86.7 and 30.4 mg/g at pH 4.0 and 5.5, respectively. The experimental equilibrium data fitted well to the Temkin isotherm model. Finally, the hydrazine functionalized poly(VBC-g-
GMA
) beads were used for the purification of
invertase
from crude yeast extract in a batch system and the purity of the eluted
invertase
from the hydrazine functionalized beads was determined as 92% by HPLC from single step purification protocol.
...
PMID:Poly(glycidylmethacrylate) brushes generated on poly(VBC) beads by SI-ATRP technique: hydrazine and amino groups functionalized for invertase adsorption and purification. 1934 18
