Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:3.2.1.26 (invertase)
 4,927 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Leucrose [D-glucosyl-alpha(1----5)-D-fructopyranose] prepared by microbial-enzymatic transglycosidation from sucrose, is the first alpha(1----5)-linked disaccharide which possesses excellent nutritional properties with regard to metabolic utilization and is well tolerated. The aim of the present work was to assess its cariogenic potential. Yeast invertase was shown to be inhibited by leucrose in a noncompetitive way, while hydrogenated leucrose (leucritol) acted as an activator. Plaque polysaccharide forming glucosyltransferases from Streptococcus cricetus AHT were not influenced by leucrose, but by leucritol. Essentially no acid formation was observed after incubation of leucrose with suspensions of human dental plaque, S. mutans NCTC 10449, Lactobacillus casei LSB 132 and Actinomyces viscosus Ny 1 No. 30. Leucrose was a competitive inhibitor of the acid formation from sucrose by S. mutans NCTC 10449 at neutral pH. Furthermore, leucrose inhibited at neutral pH considerably the uptake of sucrose by S. mutans NCTC 10449. The uptake of fructose and maltose was also inhibited but that of glucose not at all. In Cara rats as the animal model, leucrose was compared to sucrose and to corn starch for its cariogenic potential. In sharp contrast to the group fed with 30% sucrose, caries scores of the 30% leucrose group were not significantly different from the starch group. pH telemetry with an indwelling electrode in man proved lueucrose to be 'safe for teeth' since plaque pH did not drop below pH 5.7. Leucrose is a novel noncariogenic disaccharide and thus represents a highly promising sugar substitute for caries prevention.
Caries Res 1989
PMID:Cariological assessment of leucrose [D-glucopyranosyl-alpha(1----5)-D-fructopyranose] as a sugar substitute. 250 93

The effect of sucrose rinses on the salivary microflora and on bacterial sucrose cleaving, i.e., sucrase activity, was studied in 11 dental students with high salivary counts of mutans streptococci (> or = 10(5) CFU/ml). The subjects rinsed for 1 min with a 10% (w/v) sucrose solution every 4-h during waking hours on 2 consecutive days. Four paraffin-stimulated saliva samples were collected as follows: one before the rinses and then 12 h and 5 and 8 days after the rinses. Commercial dip-slide methods were used to determine viable counts of mutans streptococci, lactobacilli, and yeasts; mutans streptococci were also enumerated using MSB agar, while blood agar was used to recover the total facultative and anaerobic bacteria. The sucrase activity was determined using both whole (mainly cell-associated) and centrifuged (extracellular) saliva samples. The proportions of facultative bacteria (p = 0.007) and mutans streptococci (p = 0.001) increased as a result of the sucrose rinses. No changes were detected in the number of lactobacilli or yeasts. The sucrose rinses increased both the cell-associated (p = 0.049) and the extracellular (p = 0.011) sucrase activities. The measurement of sucrase activity may be of value in assessing dietary habits and sucrose consumption.
Caries Res 1993
PMID:Effect of sucrose rinses on the oral microflora and on salivary sucrase activity. 844 72

Samples of whole saliva and dental plaque were collected from initially 10-year old subjects who participated in a 40-month cohort study investigating the effect of chewing gum usage on caries rates. The subjects represented nine cohorts of which one did not receive gum, while in eight cohorts the subjects received gum containing either xylitol, sorbitol, their mixtures, or sucrose as bulk sweeteners, the maximum sweetener consumption in the form of gums being up to 10.7 g/day, used in 3-5 daily chewing episodes. Gum usage had no significant effect on the levels of salivary protein, IgA, alpha-amylase, peroxidase, lysozyme, SCN and buffer capacity. At the endpoint, the group that received 100% xylitol pellet-shaped gum five times/day, had significantly lower levels of sucrase (p <0.05) and free sialic acid (p < 0.001) in whole saliva than at baseline. This group showed significantly (p <0.05) smaller plaque index scores at two cross-sectional measurements, and exhibited the lowest log(10) counts of salivary lactobacilli at endpoint than most other groups. The salivary levels of peptidase(s) (oligopeptidase B-like enzymes) hydrolyzing N-alpha-benzoyl-DL-arginyl-p-nitroaniline were significantly (p<0.05) or almost significantly lower in groups which received 100% xylitol pellet gums. All groups exhibited obviously an aging-related increase of salivary mutans streptococcus scores, except the above xylitol group in which the mean scores did not change.
Caries Res 1996
PMID:Properties of whole saliva and dental plaque in relation to 40-month consumption of chewing gums containing xylitol, sorbitol of sucrose. 886 27